Abstract: This study was designed to carry out the molecular identification of clinical isolates of Mycoplasma ovipneumoniae(Mo)prevalent in Anhui Province by using a specific PCR assay targeting at p113 gene.PCR assay was used to amplify the target gene from the four previously isolated Mo strains,AH-01,AH-02,AH-03 and AH-04,using the reported specific primers for p113 gene.Subsequently,the amplified products were sequenced and genetically analyzed.The results showed that the specific PCR products with size of 287 bp were obtained from all of the four clinical isolates;the pairwise sequence similarity of p113 gene of the four Mo isolates were all above 95%,and their sequence similarity with Mo standard strain of ATCC 29419 and Mo clinical strain of GZ-QX1 isolated from Guizou Province ranged from 88.6% to 89.3%.Our results demonstrated that the PCR assay targeting at p113 gene could be used in the molecular identification of Mo clinical isolates,which provided a methodological reference for the epidemiological investigation and scientific control for the respiratory tract infectious diseases in sheep and goat associated with Mo.