马丙酮酸脱氢酶激酶4基因序列生物信息学分析

doi:10.12160/j.issn.1672-5190.2019.11.001

Animal Husbandry and Feed Science ›› 2019, Vol. 40 ›› Issue (11): 1-6.doi: 10.12160/j.issn.1672-5190.2019.11.001

Bioinformatical Analysis of Horse Pyruvate Dehydrogenase Kinase 4（PDK4）GeneSequence

CHEN Jian-xing¹, MA Zhi-yan¹, LIU Yong-bin², LIU Juan¹, DONG Yan-fei¹, SUN Yu-jiang^3,4

1.College of Chemistry and Life Science,Chifeng University,Chifeng 024000,China;
2.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
3.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;
4.Vocational College of Dongying,Dongying 257091,China

Received:2019-08-14 Online:2019-11-30 Published:2019-12-19

Abstract

Abstract: In order to reveal the coding characteristics and the secondary structure of pyruvate dehydrogenase kinase 4 （PDK4） gene in horse, the gene sequence of horse PDK4 was analyzed by using bioinformatical software. The results showed that in the CDS region of horse PDK4, the most frequently observed codons were AUU, AUG and GAU （14 times）, accounting for 34‰ of all the codons （413）. The minimum RSCU value （0） was found in UGC codon which was not involved in the coding region, the maximum RSCU value （2.22） was observed in the ACA codon, and the RSCU value of the five codons of CAU, CAC, CGA, AAA and AAG was 1, indicating no codon usage bias was found in these codons. A total of 412 amino acids were translated in the CDS region of horse PDK4, with the highest proportion of leucine and the lowest proportion of tryptophan. The complicated secondary structure of the horse PDK4 protein was dominated by coils, and β-strands as well as α-helices were complementary. The distribution of hydrophilic amino acids in horse PDK4 protein was more frequently observed than that of the hydrophobic amino acids, and the overall hydrophobicity of PDK4 was poor. The predicted isoelectric point of PDK4 was 6.64.

Key words: creatine kinase, bioinformatical analysis, secondary structure, RSCU

CLC Number:

CHEN Jian-xing, MA Zhi-yan, LIU Yong-bin, LIU Juan, DONG Yan-fei, SUN Yu-jiang. Bioinformatical Analysis of Horse Pyruvate Dehydrogenase Kinase 4（PDK4）GeneSequence[J]. Animal Husbandry and Feed Science, 2019, 40(11): 1-6.

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Bioinformatical Analysis of Horse Pyruvate Dehydrogenase Kinase 4（PDK4）GeneSequence

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