Abstract: In order to investigate the function of porcine group A rotavirus VP7 gene, a set of primers were designed based on the DNA sequence of porcine rotavirus VP7 gene in GenBank. The rotavirus Shanghai isolate SH-1 was used as a template for PCR amplification, and the obtained VP7 gene was cloned into pMD-18T vector for sequencing and analysis. The results showed that the VP7 gene sequence encoded 326 amino acids with relative molecular weight of 37.38 Ku and theoretical isoelectric point (PI) of 4.77. The VP7 protein was dominated by alpha helices and contained 13 epitope regions. Phylogenetic tree analysis showed that the isolate SH-1 was genetically closest to KM230930.1 strain. The genotype analysis demonstrated that the isolate SH-1 was G10 type which belonged to the zoonotic virus strain.

Key words: group A rotavirus, VP7 gene, cloning, bioinformatical analysis