Abstract: [Objective] To compare the disinfection effects of hydrogen peroxide and formaldehyde on the interior surface, mesa and air of the breeding room of barrier SPF rats and mice, and to select effective disinfectants. [Method] The disinfection effects of hydrogen peroxide and formaldehyde by fumigation on reproductive chambers of SPF rats and mice were observed via air falling bacteria detection method and swab method. [Result] After fumigation with hydrogen peroxide and formaldehyde, the bacteria in the air of SPF rats breeding room and mice breeding room were detected. The sterilization rates of hydrogen peroxide and formaldehyde in the air of rats breeding room and mice breeding room were 100%, 97.5% and 100%, 100%, respectively. The bacterial colonies on the walls, floors and cage surfaces were detected for peroxidation. The bactericidal rates of hydrogen peroxide and formaldehyde on the walls, floors and cage surfaces of rats reproductive chambers were 100%, 99%, 100% and 95%, 100%, 99%, respectively, and those of mice reproductive chambers were all 100%. [Conclusion] Steam disinfection with hydrogen peroxide is an ideal disinfection method, which can effectively control the microorganisms in the breeding room of SPF rats and mice and has high safety and obvious disinfection effect.

Key words: hydrogen peroxide, formaldehyde, SPF rats and mice, breeding room, disinfection