肉牛粪污中纤维素降解菌的分离筛选

doi:10.12160/j.issn.1672-5190.2019.11.019

Animal Husbandry and Feed Science ›› 2019, Vol. 40 ›› Issue (11): 79-83.doi: 10.12160/j.issn.1672-5190.2019.11.019

Isolation and Screening of Cellulose-degrading Bacteria from the Manure of Beef Cattle

QIAO Jian-min¹, YUE Lin-fang¹, CHENG Li-xin¹, ZHENG Zhong², LI Zi-jian¹, Fengying¹, LI Yun-hua¹, WANG Zhi-ming¹, Baohua¹, YU Zhao-hui¹

1.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
2.Inner Mongolia University,Hohhot 010021,China

Received:2019-10-08 Online:2019-11-30 Published:2019-12-19

Abstract

Abstract: This study aimed to obtain the bacterial strains that could effectively degrade the cellulose in beef cattle manure. The cellulose-degrading bacteria were isolated from cattle feces and their natural compost samples by using sodium carboxymethyl cellulose as carbon source. Subsequently, the isolated strains were morphologically and molecularly identified. The cellulose-Congo red medium and filter paper degradation test were used to further assess the cellulose-degrading ability of the isolates. The results showed that a total of 32 cellulose-degrading isolates were obtained, and 4 of them （designated as XQ-1, XQ-2, XQ-3 and XQ-4, respectively） had hydrolyzed circle in Congo red medium and had a certain ability to degrade the filter paper. The 16S rDNA sequencing identification demonstrated that the 4 strains were Escherichia coli, Serratia marcescens, Providencia rettgeri and Escherichia fergusonii, respectively.

Key words: beef cattle manure, cellulose-degrading bacteria, isolation and screening

CLC Number:

X713
S141.2

QIAO Jian-min, YUE Lin-fang, CHENG Li-xin, ZHENG Zhong, LI Zi-jian, Fengying, LI Yun-hua, WANG Zhi-ming, Baohua, YU Zhao-hui. Isolation and Screening of Cellulose-degrading Bacteria from the Manure of Beef Cattle[J]. Animal Husbandry and Feed Science, 2019, 40(11): 79-83.

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Isolation and Screening of Cellulose-degrading Bacteria from the Manure of Beef Cattle

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