Abstract: The CRISPR/Cas9 is a new type of RNA-targeted gene editing system. In recent years, the CRISPR/Cas9 gene editing technology has gained rapid development. Due to its economical, efficient and convenient features, CRISPR/Cas9 gene editing technology is widely used in breeding, medicine, industry and other fields. This paper introduces two types of newly developed gene editing technologies, elaborates the structure and function of CRISPR/Cas9, expounds the principle of CRISPR/Cas9 gene editing technology, and summarizes the application of this technology in forage grass, in hoping to provide references for investigating the forage grass gene function and improving forage grass traits by applying CRISPR/Cas9 gene editing technology.

Key words: CRISPR/Cas9, gene editing, forage grass, application