Abstract: [Objective] To analyze the polymorphism of c.A99G mutation site on exon 1 of adiponectin gene （NC_006096） of Wuding chicken. [Method] The whole blood samples of 67 Wuding chickens were collected from the wing veins, and the genomic DNA was extracted. The PCR products digested with Hsp92Ⅱ restriction enzyme were analyzed via PCR-RFLP assay. [Result] Three genotypes of AA, AB and BB were observed at the synonymous mutation site of c.A99G, in which the frequency of A gene was 0.686 6 and that of B gene was 0.313 4; the PIC was 0.337 8, indicating that the site was a moderate polymorphic site;χ² fitness test showed that the adiponectin gene c.A99G site was in equilibrium. [Conclusion] The PCR-RFLP method effectively detected the c.A99G mutation of adiponectin gene of Wuding chicken. There were 3 genotypes at this locus, and the alleles were at the level of moderate genetic polymorphism and were in equilibrium.

Key words: Wuding chicken, adiponectin gene, PCR-RFLP