Abstract: [Objective] To evaluate the killing effect of microcurrent against bacteria in water body, and to provide basis for developing new disinfection techniques for livestock and poultry sewage. [Method]Suspension quantitative bactericidal test, influencing factors determination test and simulated field test were conducted to assess the killing effect of microcurrent against Escherichia coli （E. coli）, Staphylococcus aureus （S. aureus）, Pseudomonas aeruginosa （P. aeruginosa） and Bacillus subtilis （B. subtilis）, to evaluate the impact of temperature and pH value on disinfection effect, and to investigate the disinfection effect of microcurrent on artificial lake water and waste water from livestock and poultry farms.[Result]The killing log values against the above 4 bacterial suspension were all higher than 5.0 after the treatment of microcurrent for 4 min. Temperature and pH value had no impacts on disinfection effect of microcurrent. After the treatment of microcurrent for 4 min, 99.99% of the total bacteria in the artificial lake water and waste water from livestock and poultry farms were killed, indicating that the bacteria in the water samples were almost killed. When treatment duration extended to 5 min, the total number of bacterial colonies in the water samples were reduced to 0 CFU/mL, indicating that the bacteria in the water samples were completely killed. [Conclusion]Microcurrent has killing effect against the commonly observed bacteria in livestock and poultry sewage, and has broad application prospect in disinfection in livestock and poultry rearing industry.

Key words: microcurrent, bacteria, killing effect, influencing factors