Animal Husbandry and Feed Science ›› 2022, Vol. 43 ›› Issue (3): 36-41.doi: 10.12160/j.issn.1672-5190.2022.03.006

• Animal Nutrition and Feed Science • Previous Articles     Next Articles

Optimization of Preparation Conditions of Ethanol Extracts from Artemisia scoparia

GUO Chen-yang1,2, LIU Jia-lin1,2, ZHONG Hua-chen1,2, WANG Li-fang1,2   

  1. 1. Agricultural Product Quality Safety Risk Assessment Laboratory(Hohhot),Ministry of Agriculture and Rural Affairs,Hohhot 010031,China;
    2. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2022-02-21 Published:2022-05-24

Abstract: [Objective] To determine the optimal preparation conditions of ethanol extracts from Artemisia scoparia, a compositae plant. [Method] The extracts from Artemisia scoparia were prepared by the condensation tube reflux extraction method with ethanol as extraction solvent. A orthogonal test was designed by four factors (extraction temperature, ethanol concentration, solid-liquid ratio, and extraction time) with three levels respectively. The optimal preparation conditions were determined according to the yield of ethanol extracts. [Result] According to the analysis of variance and range R value, orders of the extraction effects of the four factors on yield of ethanol extracts of Artemisia scoparia was as follows, extraction temperature > ethanol concentration > extraction time>solid-liquid ratio. The influence of extraction temperature on yield of ethanol extracts was extremely significant (P<0.01), revealing that it was the primary factor impacting the extraction effect. The ethanol concentration, solid-liquid ratio, and extraction time had no significant (P>0.05) effects on yield of ethanol extracts, demonstrating that they were the secondary variables impacting the extraction effect. The optimal preparation conditions of ethanol extracts from Artemisia scoparia were ethanol concentration at 65% with a 1∶7 solid-liquid ratio, and 3.0 h of extraction at 75 ℃. [Conclusion] This study determined the optimal preparation parameters for ethanol extracts from Artemisia scoparia under laboratory conditions, providing a reference for further research and application of extracts and bioactive components of Artemisia scoparia.

Key words: Artemisia scoparia, ethanol extracts, yield, orthogonal test, preparation condition

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