Abstract:

[Objective] The P113 genes of Mycoplasma ovipneumoniae isolates from Inner Mongolia were cloned, and the P113 protein sequences of various isolates were bioinformatically compared and analyzed, so as to provide references for clarifying the biological function of Mycoplasma ovipneumoniae P113 protein. [Method] A set of specific primers were designed to amplify the P113 gene fragments of 9 isolates of Mycoplasma ovipneumoniae from Inner Mongolia. The obtained P113 gene fragments of various isolates were sequenced. The DNA sequences were then translated into amino acid sequences, and the differences in amino acid sequences among various isolates were bioinformatically compared. [Result] The PCR amplified products of P113 gene of various isolates varied in size. According to amino acid sequence analysis, the lengths of C-terminal repetitive elements were variable. KKAEGA (S) QNQG was the dominantly observed repetitive element. The number of repetitive elements of the isolates varied. The most repetitive elements were found in the NM01-MO isolate and CK-MO isolate, both of which were 16. The least repetitive elements were found in the LK-MO isolate, which were 3. Most isolates had large differences in the number of repetitive elements. [Conclusion] The number of C-terminal repetitive elements in the amino acid sequence of P113 protein of Mycoplasma ovipneumoniae isolates from Inner Mongolia was variable. These repetitive elements had an impact on P113 protein structure, which could then have an impact on its biological activity.

Key words: Mycoplasma ovipneumoniae, P113 gene, P113 protein, repetitive elements, biological function