新疆维吾尔自治区喀什地区某引种奶牛场新生犊牛的牛轮状病毒分子检测及遗传特征分析

doi:10.12160/j.issn.1672-5190.2025.01.015

Abstract

Abstract: [Objective] To investigate the infection status and molecular genetic characteristics of bovine rotavirus in neonatal calves from an introduced dairy farm in the Kashgar region of the Xinjiang Uygur Autonomous Region. [Methods] Fecal samples were collected from 50 calves under three months of age at the dairy farm in Kashgar. The presence of bovine rotavirus was detected using RT-PCR, and the resultant sequences were subjected to genotype determination and phylogenetic analysis. [Results] Based on VP6 gene detection, 6 tested positive for bovine rotavirus, yielding an infection rate of 12.00% （6/50）. Sequence analysis of the VP7 gene from the six positive samples revealed two genotypes: G6 （n=4） and G8 （n=2）. Analysis of the VP4 gene indicated that all 6 positive samples belonged to the P[5] type. Consequently, two bovine rotavirus genotypes were identified at the dairy farm, namely G6P[5] （n=4） and G8P[5]（n=2）. Phylogenetic analysis demonstrated that the G6 genotype was closely related to bovine rotavirus strains from Turkey, whereas the G8 genotype showed closer genetic proximity to human rotavirus strains from Japan; the P[5] genotype was most similar to bovine rotavirus strains from Iran. [Conclusion] The bovine rotavirus detected in the calves of this dairy farm exhibits considerable genetic diversity, with the G6P[5] genotype being predominant. The study is the first to report the presence of the G8P[5] genotype in bovine rotavirus in the Kashgar region of Xinjiang, thereby providing valuable insights for the prevention and control of bovine rotavirus infections in southern Xinjiang.

Key words: rotavirus, detection, identification, genetic evolution, cattle

CLC Number:

LI Ping, ZHANG Bowen, ZHU Yanan, SU Duoduo, LI Jing, JING Bo, ZHANG Zhenjie, QI Meng. Molecular Detection and Genetic Characterization of Bovine Rotavirus in Neonatal Calves from an Introduced Dairy Farm in the Kashgar Region of the Xinjiang Uygur Autonomous Region[J]. Animal Husbandry and Feed Science, 2025, 46(1): 114-119.

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Molecular Detection and Genetic Characterization of Bovine Rotavirus in Neonatal Calves from an Introduced Dairy Farm in the Kashgar Region of the Xinjiang Uygur Autonomous Region

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