Abstract: [Objective] To explore the effects of selenium-enriched black soldier fly on growth performance, serum biochemical indices, antioxidant indices and tissue selenium content of Micropterus salmoides. [Methods] A single-factor completely randomized experimental design was adopted. A total of 270 juvenile M. salmoides with an average weight of （50.31±0.21） g were selected and randomly divided into 3 groups, with 3 replicates in each group and 30 fish in each replicate. The control group （CN） was fed a basal diet supplemented with non-selenium-enriched black soldier fly; the sodium selenite group （SS） was supplemented with 0.30 mg/kg selenium on the basis of the control group; the selenium-enriched black soldier fly group （Se-BSF） was fed a basal diet supplemented with 2% selenium-enriched black soldier fly. The experiment lasted for 60 days. Growth performance data were recorded before and after the experiment. Blood, liver, muscle, and whole-body samples were collected to determine serum biochemical indices, antioxidant indices, and selenium content in each tissue. [Results] In terms of growth performance, compared with the control group （CN）, the final body weight, specific growth rate and feed efficiency of M. salmoides in the selenium-enriched black soldier fly group （Se-BSF） were significantly improved （P<0.05）; other indices showed improvement trends but no significant differences （P>0.05）; compared with the control group （CN） and the selenium-enriched black soldier fly group （Se-BSF）, there was no significant difference in all indices in the sodium selenite group （SS） （P>0.05）. In terms of serum biochemical indices, compared with the control group （CN）, the total protein （TP）, albumin （ALB） and high-density lipoprotein cholesterol （HDL-C） in the serum of M. salmoides in the selenium-enriched black soldier fly group (Se-BSF) were significantly increased （P<0.05）; the serum triglyceride （TG） and low-density lipoprotein cholesterol （LDL-C） of M. salmoides in the selenium-enriched black soldier fly group (Se-BSF) were significantly （P<0.05） lower than those in the sodium selenite group （SS） and the control group （CN）. In terms of serum antioxidant indices, compared with the control group (CN), the total antioxidant capacity （T-AOC）, total superoxide dismutase （T-SOD） and glutathione peroxidase （GSH-Px） in the serum of M. salmoides in the selenium-enriched black soldier fly group （Se-BSF） were significantly increased （P<0.05）; the serum malondialdehyde （MDA） of M. salmoides in the selenium-enriched black soldier fly group （Se-BSF） was significantly （P<0.05） lower than that in the sodium selenite group （SS） and the control group （CN）. In terms of tissue selenium content, compared with the control group （CN）, the selenium content of muscle, liver and whole body of M. salmoides in the selenium-enriched black soldier fly group （Se-BSF） were significantly increased （P<0.05), and the selenium content in each tissue in the sodium selenite group （SS） showed an increasing trend, but the difference was not significant （P>0.05）; there was no significant difference between the selenium-enriched black soldier fly group （Se-BSF） and the sodium selenite group （SS） （P>0.05）. [Conclusion] Dietary supplementation with selenium-enriched black soldier fly can significantly enhance the growth performance of M. salmoides, improve serum biochemical indices, strengthen the body′s antioxidant capacity, and effectively promote the deposition of selenium in various tissues. This provides a reference for the healthy breeding of M. salmoides and the development of high-quality selenium-enriched products.

Key words: Micropterus salmoides, black soldier fly, selenium, growth performance, serum biochemical indices, serum antioxidant indices