Abstract: First, PCR primers were designed according to the complete sequences of bovine herpes virus type l of GenBank, then genomic DNA of the IBRV NM06 strain isolated in Inner Mongolia was extracted, and the gene was amplified by PCR. Second, PCR products were cloned and sequenced. The result showed that the length of gE gene was 1 793 bp among the sequence of 2 086 bp including the whole open reading frame of 1 728 bp, and its amino acids was 576. The result of Blast showed that gE gene of NM06 strain in homology was ranged from 99.3 % to 99.9 % compared with four strains of bovine herpes virus 1.1 （BHV-1.1）, the homology of nucleotide sequences of gE gene among NM06 strain with two strains of BHV-I.1 were 99.5 % and 99.6 % respectively. In phylogenetic tree, NM06 strain and BHV-I.1 virus strain belong to the same evolutionary branch, but BHV-1.2 BH83 strain belongs to another evolutionary branch, therefore IBRV NM06 isolates was presumed as type of BHV-l.lbovine herpes virus 1.1. In addition, nucleotide sequences of gE gene of NM06 strain in homology was 82.9 % compared with bovine herpes virus type 5 N569 strain, and 72.6 % compared with Cervid herpes virus type2 Norway 4.