RNA interference is an evolutionarily conserved defense mechanisms to resist transgenic genes and alien virus, it lies in many different species of organisms, and can transfer between organisms cells with the effect of resisting virus invasion and maintaining genome stahility. RNA interference library is based on RNA interference technology with the feature of the simplicity, efficiency, large- scale and high throughput, can be used in large scale screening of gene function and phenotype screening. Research and application of RNA interference as an extremely important theoretical and practical significance of the medical biology will have a profound impact on development.

The production records of 21 569 Arbas cashmere goats during from 1997 to 2007 were collected, the variance analysis was made by using GLM program of SARS 9.0 software, and multiple comparison of main effect was carried out with Duncan methods to determinate effects of nine non-genetic factors （tested year, sex, age, mother age, birth type, birth month, weaned age, birth order and field） on 5 traits of producing cashmere （cashmere yield, cashmere fineness, body weight, cashmere and length）. The results indicated that there were significant effects of the tested year, field, age and mother age on the all traits of cashmere goats （P〈0.05）. Except for cashmere fineness, the sex significantly affected the cashmere yield and body weight after producing cashmere （/9〈0.05）, specifically it had markedly significant effects on cashmere thick and wool length（P〈O.01 ）. The birth order had a significant effects on cashmere yield, wool length and early growth traits （P〈0.05）; the birth type had a significant effect on degree of cashmere fineness and body weight after producing cashmere （P〉0.05）; the birth month had a significant influences on cashmere yield（P〈0.05 ）.

The main physiological and biochemical indexes of 30 adult Cashmere goats from Zhandanzhaosumu were determined in the trial. The results showed the contents of RBC, WBC and Hb respectively were 14.80×10^12/L, 10.81×10^9/L, 81.73 g/L. The contents of Lymphocyte,Basophil,Acidophilic cell and monocytes respectively were 56.13 %, 0.64 %, 4.50 %, 3.61%; The contents of TP, ALB, serum globulin （G）, A/G, BUN, GOT, GPT and AKP respectively were 54.28 g/L, 34.57 g/L, 19.70 g/L, 2. 13 g/L, 2.64 mmol/L, 53.94 IU/ L, 19.79 IU/L and 54.26 U/100 mL.

The insulin-like is one of the main mediate factors for growth hormone, and can promote animal growth. By systematically explaining the function and mechanism of action of the IGF- I gene, it revealed it is important in the process of animals growth and development.

Based on comparative study and concrete practice, standard methods for determining laccase, manganese peroxides and lignin peroxidase activities were introduced in the article, and their concrete operation steps were also described in detail. In addition, analytical application of the standard methods on practical research was also summarized.

Sequence analysis was conducted on genes of omp31 from 3 different Brucella strain isolated from Inner Mongolia. A pair of primers were designed for amplification of Brucella omp31 genes according to the reported of Brucella omp31 genes complete sequence. omp31 genes were amplified from 3 different Brucella strains by polymerase chain reaction （PCR）, and the PCR products were cloned into pMDI9-T vector and sequences were analyzed. The results showed nucleotide sequences in homology among 3 different Brucella isolates omp31 genes were 100.0 %. Nucleotide sequence in homology was 100.0 % between the three isolates genes and Brucella suis 1330, Brucella ceti B14/94 and Brucella neotomae 5K33, its homology was 99.0 % compared with omp31 gene of Brucella canis RM6/ 66, Brucella melitensis 183 and Brucella melitensis 16M, but the homology between the obtained and Brucella ovis ATCC25840 was the lowest, only 98.8 %.

The experiments were designed for producing high protein and low cellulose feed of straw by muhiple strains fermentation. Two strains of white rot fungi were used to ferment straw, the crude protein in the fermentation was detected by Kjeldahl method and the crude fat was detected by Soxhlet extraction method. The results indicated that the crude protein reached 21.87 % by the mixed fermentation, the primary culture medium of cornmeal was better than others. The experiment of fermentation had provided scientific： proofs for production of crop feed.

First, PCR primers were designed according to the complete sequences of bovine herpes virus type l of GenBank, then genomic DNA of the IBRV NM06 strain isolated in Inner Mongolia was extracted, and the gene was amplified by PCR. Second, PCR products were cloned and sequenced. The result showed that the length of gE gene was 1 793 bp among the sequence of 2 086 bp including the whole open reading frame of 1 728 bp, and its amino acids was 576. The result of Blast showed that gE gene of NM06 strain in homology was ranged from 99.3 % to 99.9 % compared with four strains of bovine herpes virus 1.1 （BHV-1.1）, the homology of nucleotide sequences of gE gene among NM06 strain with two strains of BHV-I.1 were 99.5 % and 99.6 % respectively. In phylogenetic tree, NM06 strain and BHV-I.1 virus strain belong to the same evolutionary branch, but BHV-1.2 BH83 strain belongs to another evolutionary branch, therefore IBRV NM06 isolates was presumed as type of BHV-l.lbovine herpes virus 1.1. In addition, nucleotide sequences of gE gene of NM06 strain in homology was 82.9 % compared with bovine herpes virus type 5 N569 strain, and 72.6 % compared with Cervid herpes virus type2 Norway 4.

According to the application of 1,4-dihydropyridinne on livestock and poultry in recent years, physiological function and effects of 1,4-dihydropyridine on production performance, reproduction performance, heat stress of livestock and poultry were reviewed to improve the utilization of 1,4-dihydropyridine in the article.

Two key bioprocesses including ruminal bio-hydrogenation of fat and fatty acids synthesis in mammary, affect compositions of milk fatty acids, were analyzed, and fatty acids composition and characteristics of different forages were discussed in detail. Moreover, the effect of different dietary forages on milk fatty acids composition was particularly reviewed.

he ruminant fermentation has been increasingly paid attention to, thus many methods were produced. In the article, several methods on ruminant fermentation in vitro were reviewed including gas production technique, two stage method and continuous culture in vitro.

Due to the importance of dietary lipid utilization for larval rearing success, increasing attention has been paid during the last years. By reviewing digestion, absorption, transport and metabolism of lipid nutrition in larval rearing, the aim was to better understand larval lipid nutrition and promote the development of fishery.

This experiment was conducted to study the effects of milk replacer treated by expanding technology on growth performance and development of digestive organ in calves, and provide theoretical basis in production practice. 20 newly born calf were randomly divided into two groups with the approimate principle in body weight and birth, namely the trial group（TP） and the control group（CP）. The test group started has been foraged training after they birthed 6 d until 20 days, then the milk replacer was used as the only feed sources for the trail group. Meanwhile, milk was also the single food for the control group, the trial duration lasted 60 d. The results showed that every calf can be saved for 315 kg milk from birth to weaning; Average body weight and daily weight gain of calves fed milk replacer were better than that of the control group, but there was no difference between them （P〉0.05）. From the 40th day of experimental period, the body height, body slanting length, chest girth of TP were significantly greater than that of CP （P〈0.05）. Diarrhea rate in TP decreased 13.16 %. Fed the milk replacer had a positive effect on growth and development of small intestine, esophagus, rumen, reticulum, omasum, abomasum.

As a feed additive, probiotics has become the focal point of research.Its varieties, functional mechanism and application effect in poultry feed as well as points for attention are overviewed in the article, meanwhile its application prospects also are put forward.

The present situation of experimental teaching in Medicine of Pets was analyzed, its significance and aim was put forward, and specific practice was also summarized in the article. Synthetic experiment can be realized by reasonably integrating many trials into one experiment, and it also meets with the demands of diagnosis and treatment in clinic. Realization of synthetic experiment in Medicine of Pets will be useful to improve student＇s ability of thinking, their interests, positivity and disposition. Meanwhile, it can also expand the content of teaching, it takes significance on talented man culture of creation and improvement of teaching ability to teacher.

Lab construction is important for talent cultivation and scientific research in universities, but the currently experimental talent teams has seriously hampered the long-term sustainable development of universities. By analyzing the present status of laboratory talent team, some measures and proposals for construction of laboratory teams were put forward to provide the reference to long-term development of laboratory in this paper.

In the thesis, based on teaching experience for many years, teaching emphases of Animal Nutrition and Feed Science in different specialties including the points of theory and practices were explored combining with the features of different specialties, and we hoped that reference couhl be offered to fraternity for their teaching and making of syllabuses.

As one of the important part of clinic teaching for animal medicine, the objective of practice teaching is to make students touch and know the common disease and frequently encountered disease from different pets and get training in basic skill in clinic, and make them master working program and the common sense of treatment. According to the existing problems in practice, the organization and practice of project were improved to raise the effect combining with the experience of pre-training of taking the position and training of basic skill.

A nimal Reproduction is one of the most important professional courses in the first grade discipline of stockbreeding, and it is also a kind of subject with strong sense of experiment, so it needs the skill very high for students. In order to improve the practice teaching effect of the Animal Reproduction, the major problem of the practice teaching was analyzed in the paper, and some reforming countermeasures were given.

According to culture objective of specialty school and the psychological feature of students, the teaching concept got shifting in Xinyang Agricultural College, and the principle of reform and practice, select of teaching content and compilation of teaching materials were reformed. Based on the above construction, the teaching system of Animal Anatomy has been basically set up and improved with exploration and summary for many years, and it formed the vivid feature in high agricultural specialty school.

The prueess management is an important part of management of experimental teaching. Preparation in teaching material and experimental condition takes effect on the realization of teaching objective, keeping the coincidence in explanation and emonstration in teaching means, process and direction, cleaning and sorting in material, writing anti correcting in experimental report is key to guarantee quality of experimental teaching. The quality appraisals take effects of diagnosis and direction to experimental teaching. The preparation. organization and enforcement, quality appraisal of experimental teaching was discussed and attempted in this paper.

The development of diagnostic method of canine distemper（CD ） in cell biology and molecular biology was summarized in this paper. In the cell biology aspect, the diagnosis of CD was mainly depended on virus isolation and culture,artificial replication experiment, election microscope examination, inclusion body examination, specific antigen and antibody examination and so on. Along with the development of molecular biology, nucleic acid hybridization and RT-PCR diagnosis of canine distemper virus （CDV） have been established. These methods in sensitivity and specificity were higher compared with the traditional methods, especially in the earlier period of CDV infection when the animal body can＇t develop an immune response.

In order to establish rapid testing methods for piglets eperythrozoonosis, pathological anatomy and pathohistology was applied to examine piglets suffering from eperythrozoonsis in this experiment. The results indicated that it caused cell degeneration and necrosis in liver, spleen, lymph node of piglet infected with Eperythrozoon, meanwhile also a lot of red cells splintered. The conclusion could be made that massive destruction of red blood cells and liver, cell degeneration of spleen and lymph node and necrosis is the main cause of the deaths of piglets.

To master morbidity rate of Demodex canis in Zhengzhou City, many cases data from animal hospitals was collected from 2008, 7 to 2009, 1, and the suspected cases was examined in microscope, 118 cases among them were diagnosed, morbidity time and location, varieties, sexuality and age were analyzed. The results showed that the morbidity rate of the disease during from July to October was higher than that of others, the symptom in abdominal part and cervical part was the most significant, then limbs, pinna, head and dactyl. Hybrid eannie and Jingba were the easiest to be infected, then Jinmao, Songshi, Bomei and Labuduo. In age, 1-2 age cannie was easier to be infected. In sexuality, morbidity rate in male was higher than that of female.

Hepatitis E was caused by Hepatitis E virus possessing the similar symptom with Hepatitis A. Nowadays, five gene types were found with distribution of regionalism, mainly in developing countries among Asia, Africa and Central America. HEV is transmitted via the faecal-oral route and close to human being and animals, can do damage to human being and animals. In the article, the brief review on its epidemiology was made to provide reference for prevention of it.

As one of the common poultry disease, pullorum disease is caused by Salmonella and does great loss to poultry industry. Different varieties all were easy to be infected, the morbidity and mortality rate among 2 - 3-week-old chicks are the highest, and it can do serious damage to adult chicken＇s reproductive system resulting in decrease of egg laying and rooster sexual dysfunction. The whole blood plate agglutination test was taken to detect its antibody titer of 200 roosters at X chicken field of Helan County in Ningxia Province.＇ The result showed that there were 11 positive of them, and the morbidity rate was 5.5 %.

Veterinary public hygiene is composited of zoonosis, animal food hygiene, protect environment, comparative medicine and basic medicine, thus it is the important part of existing safety of human being and can＇t be replaced by others.

Polycyclic aromatic hydrocarbons （PAHs） showed high carcinogenicity and the tendency of continuous increase in environment, its main degradation path was completed by microorganism. The styles of PAHs degradation by microorganism and research progress at home and abroad on PAHs degradation by bacterium, fungi and the other microorganism were reviewed in the article, its applicable prospect also was discussed.

The resources of Pheasant in Mudanjiang areas were investigated to better protect and make use of them in the study. From July, 2007 to December, 2009, the survey of the dense of Pheasant population in Mudanjiang areas was made by fixed route trip method and point method. The results showed that there were 4 Pheasant species in total were found, Phasianus colchicus is superior amounting to 46.2 % in all Pheasant population, its average dense was 1.8 per square kilometer, but Coturnix coturnix was rare, only amounting to 9.23 %. The result also showed that distribution of Pheasant is closely related with surroundings, the number of Pheasant in grasslands and bushes in meadow is large in summer, but the habitat is related with the food conditions in spring and winter. The difference of distribution is also caused by human activities.

The habitat environment of amphibians was investigated at Fuxian lake＇s main inflow rivers and artificial wetland and lakeside zone to reveal the relation between amphibian and the environment. The results showed that the distribution of amphibian was narrow, the kinds of Anura abundantly exist. The quantity and kind of amphibian is plentiful in the artifieia wetland, and the quantity is vast at Luqi river and Jianshan river of inflow rivers. The habitat area of amphibian is elosely affected by human activities, the ehange has correlation with water quality and vegetation and terrain.

In this paper,a productive test of Rotary Sludge Dewatering Press is carried out. The test showed that the effect of sludge dewatering machine was related to the flocculant, the character of sludge and so on. It will provide the guidance for improving dewatering capability of Rotary Sludge Dewatering Press.

In the trial, wastes from the cattle and sheep＇s butchery in Xining region was selected as research material and investigated to reduce environmental pollution and take comprehensive utilization. The composition of wastes were analyzed, and the comparative test between natural fermentation and enzyme microorganism fermentation was made. The results showed that there were 0.83 % N, 0.23 % P205, 0.67 % K2O, 52.18 % organic matter, total amount of organic 69.59 %, 52.66 % moisture, 30.41% ash in wastes. The conclusion can be made that there were multi-nutrient elements and high organic matter content in wastes, the fermentation time can be shortened by enzyme microorganism fermentation.

Cashmere goat industry is an important constituent of Chinese animal husbandry＇. There are plenty of variety resources of Cashmere goat and the industry has developed rapidly in china. In this paper, by comparing variety characteristic at home and abroad and evaluating the production characteristics of Cashmere goat industry in China, industry status, industrial advantage and prospects of the industry in China were clarified and the developing goal and strategy of Chinese Cashmere goat industry were also proposed.

The development situation, character and the existed problems of agricultural mechanization in Anhui Province was summarized in the article, meanwhile the proposal on its promotion also and strategies for resolving theses problems was put forward.

Nowadays, the development of aquaculture insurance fall behind aquaculture in China. From the details of current status and existing problems, it is very necessary to conduct aquaculture policy insurance. It will promote the development of the aquaculture if the government or the insurance institutions provide the compensation for aquaculture producers, and patterns or models of aquaculture policy insurance also was analyzed.

The livestock industry is the important part of the national economy system, and becomes the pillar industry of agriculture and rural economic, and it makes important contribution to the food security and the construction of modern agriculture. Based on the analysis of the necessity of improving quality of livestock, the thesis puts forward specific suggestions to reinforce the livestock industry effect through improving quality, such as the industry＂ planning, adjust of structure, raising of quantity of the livestock product, building up of supervision system of livestock product, combination of the lawn resources and reasonable exploitation, to keep sustainable development.

The identification methods on artificial antigens were reviewed in the article, namely ultraviolet spectrophtonetric method, infrared spectrophotometry, electrophoresis and mass, and their advantages and disadvantages were analyzed. The conclusion can be made that mass is the best one.

Since the first cattle of in vitro production of embryo （IVP）was produced and the technology of ovum pick-up （OPU） was applied in cattle, the IVP and OPU have been greatly improved. In the article, the main studying results and situation on IVP and OPU was summarized, its application prospect was also predicted.

Oula sheep was bred in production practice by herdsman masses under the altitude of 3 800-4 500 meter for many years, it has been become the regional fine variety in the area. It is better than others with the advantages of large limbs, well-rounded back body, fast development and marked meat quality. It can get adaptation of cruelly natural environment without housing and Supplementary. Based on the many years experience, its variety resources and production performance were introduced in the article.

5 male and 5 female Wannan spotted pig were respectively selected at 0,30,45,90,180 days age, their body sizes and slaughter performance ycere detected, the data was also analyzed in the article. The results indicated that the average daily body weight again （ADBWA） impruved with the increase of day age, and the female in ADBWA after ablactation was higher than that of the male. The body height of the male at 30 days age was negatively correlate to square measure in muscular apparatus, the correlation coefficient was -0.889, but the chest measurement and the carcass plagioclase of the female at 30 days age were positively correlated to square measure in muscular apparatus, the correlation coefficients respectively was 0.942 and 0.897.

Germplasm resources and genetic diversity of horse is rich in China, and the number of the local horse reach 90 % in total. It systematic.ally reviewed varieties and diversity of germplasm resources of local horses including body conformation, chromosome, blood protein and DNA genetic diversity in the paper, and the prospect on studying their biological evolution and population genetics also was given.

Effects of different feed intake on Huangyu broilers were tested under low temperature in the trial. 432 broilers with similar body weigh at 20 week-old were randomly divided into 4 groups, and respectively fed with different diet level, namely basal diet group （C）, basal diet with 2 g feed addition （C＋2）, basal diet with 4 g feed addition （C＋4） and basal diet with 6 g feed addition （C＋6）. Statistics in egg production rate and egg weight indicated that, in the experimental period, there was no difference between experimental group and basal diet group in egg production rat （P〉0.05）, but C＋6 in egg weight was higher than that of C （P〈0.05）. However, there was no marked difference between C and the other groups in body weight again （P〉0.05）. in conclusion, application of addition into feed intake wouht improve the productivity of Huangyu broilers under low temperature to a degree.

In the trial, MTF methods was applied to examine cytotoxie activity of dandelion extract in vitro on mouse maerophages after stimulated by lipopolysaccharide （LPS）, the objective was to determine the dose of non-cytotoxicity, meanwhile nitric oxide （NO） release in LPS-stimulated maerophages was determined by Griess method to provide a basis for further study. The resuhs showed that dandelion extract is not eytotoxic to LPS-stimulated macrophages with the dosage of no more than lmg/mL. 0.25-1 mg/mL dandelion extract has obvious inhibitory effect on NO release in LPS-stimulated mouse macrophages with the relation of dose-dependent and time-dependent.

The discovery of green fluorescent protein （GFP） has greatly accelerated the development of modern biology, and it has a wide range of applications. The discovery and development process, structure, emission mechanism and application of GFP are reviewed in the article.

in this study, based on animal acute toxicity test, safe dosages of two kinds of drugs were predicted in order to provide reference for clinic. Antidote security was initially ensured with the dosage of 3 000 mg/（kg·bw） in the 1st antidote and 200 mg/（kg. bw）in the 2nd antidote by pre-test, then mice respectively was injected with the above dosage, there were no clinical symptoms and death among them. It showed that the two kinds of dosage both were safe.

This article analyzes the promotion of tumor cell apoptosis, tumor cell differentiation, tumor cell intracellular calcium concentration, the effects of tumor cell drug resistance, tumor cell proliferation, synergistically cytotoxic chemotherapy and so on, and it also elucidates the current research problems in traditional Chinese medicine drug serum.

It takes the important role in improving and enhancing immune response for adjuvant. Adjuvant not only can change immune function and recruit macrophages to swallow antigens （Ag）, but also it can change configuration of Ag, and promote its decrease and enhance its immunogenicity. Even it can extend the stored time for Ag in texture, and educe immune cells to produce synergistic effect. Based on the above mentioned, the several common adjuvant and DNA vaccine adjuvant were introduced in the article.

Sophora alopecuroides L. is poisonous perennial herb, which distribute in the desert grassland and calcareous soil or sand and farmland in our country, the main active constituent is a variety of alkaloids with a wide range of bioactive. A number of studies show that the alkaloids of S. alopecuroides L. posses multiple pharmacological effects. This article is to summarize anti-tumor effect of several common alkaloids of S. alopecuroides L. and development of their mechanism research.

The result of disinfection indicated that the disinfection effect with pre-treatment of soaking in sterile water was better than that of non-treatment, but the disinfection shouldn＇t be long after treated with soaking, the germ-carrying rate of seed was decreased greatly with pre-treatment of 75 % ethyl alcohol. Among 2 % Sodium Hypochlorite,10 % Calcium Hypochlorite and 0.1% Mercuric Chloride, 0.1% Mercuric Chloride was the best one, the germ-can＇ying rate of seed was 0 % after disinfected with 0.1% Mercuric Chloride, and the germination rate of seed also was the highest one.

To ensure normal growth and safety over-wintering of different varieties of alfalfa and improve land utilization rate and increase income, over-wintering characterization and yield from three varieties of alfalfa sowing in Autumn were investigated in the article. The result indicated that over-wintering rate of them was over 70 % with sowing on 5, August, over 50 % on 13, August, and 10.9 % -38.7 % on 20, August. Nol of Zhongmu alfalfa shouldn＇t be sowed later than in the middle of August, Jinhuanghou alfalfa should be sowed early in August, and Ningnm alfalfa should be sowed late in July.. The result also showed that all their yields of sowing on 5, August were higher than that of sowing on 5 and 13, August; in the same sowing period, the yield of No 1 of Zhongmu alfalfa among them was the highest reaching 10.49 t/hm^2, and Ningmu alfalfa was the lowest. There were markedly different in sowing period, varieties （P〈0.01 ）.

The main production performance and over-winter rate of 18 alfalfa varieties were investigated in Tongliao area in the study. The results showed that all Canada Medicago, US Medicago varia, Canada Intermediate Medicago, Aohan Medicago, U S alfalfa and Grass III were better than that of the other varieties in the main production performance and over-winter rate, especially Aohan Medicago, US alfalfa and Grass m were the optimal to suitable for planting and popularizing in the region.

By investigating, collecting, introducing and identifying, eight wild fine herbages were bred successfully. It educed active function in grassland construction, green of city and management of environment by breeding of anti-drought and anti-adversity, and the exploration, identification, evaluation, conservation and application in Genetic Resources were made positively.

Using fleshy root, young leaf and rachis of wild flower Spiranthes sinensis as explant, the rapid propagation was constructed with tissue culture in the study. The results showed the induction rate with using rachis as callus reached 83 %, the medium compositing of 3.0 %MS sucrose, 0.75 % agar, 1.0 mg/L 6-BA, 0.2 mg/L NAA and 100 g/L coconut juice was the optimization to inducing, but the optimization medium on proliferation was cumposited of 3.0 % MS sucrose, 0.75 % agar, 2.0 mg/L 6-BA and 0.l mg/L NAA. The optimization of rooting medium was composited of 2.0 % MS sucrose, 0.50 % agar, 0.5 mg/L 6-BA, 0.1 mg/L NAA and 0.5 g/L activated carbon.

Microwave digestion technique and cold atomic absorption spectrometl7 was applied to detect the total mercury in shrimp meal samples. The microwave conditions effects recovery rate and the notices were ensured, and the establishment of detection total mercury was made with microw~ive digestion-cold atomic absorption spectrometry. The recovery rate attained 98.3 %-101%, and RSD %was 1.2%-2.1%.