Abstract: VC can reduce Fe^3＋ into Fe^2＋ in acid solution, Fe^2＋ reacts with phenanthroline to form an orange complex and the speetrophotometrie signal was enhanced by [3-eyc]odextrin. According to the above results, a new method of sensitized speetrophotometry with mieellar system has been developed to analyse content of VC. Beer＇s law is obeyed in the concentration range of 0-9.8μg/mL, the apparent molar absorptivity of the complex is 1.52×10^4 L/（mol·cm）at λmax=510 nm, and it enhances 40 % compared with the absence of β-eyclodextrin.This above-mentioned method was applied to detect of the content of/VC in the fruits and vegetables, the relative standard deviations for five testes on the three kinds of fruits and vegetables respectively was 2.7, 2.8 and 2.4, and the recovery rates respectively were 95.7 %-103.1% ,96.1%-101.8 % and 96.4 %-103.5 %.