Abstract: [Objective] The aim was to study the effect of Beihanshuishi from two different places on gastric juice secretion of rat. [Method] Gastric juice secretion increasing model of rat was established by pylorus ligature method, and effects of Beihanshuishi on the gastric secretion levels, pH value and gastric acidity of rat were observed. [Result] The treated rats showed a decrease of gastric secretion levels, an increase of pH value and a decrease of gastric acidity. [Conclusion] Beihanshuishi has an inhibitory effect on gastric juice secretion of rat.