Abstract: [ Objective ] The research aimed to study the antibacterial and anti-inflammatory effect of ＂Huangjinerbai Powder＂. [Method] The anti-inflammatory test and antibacterial test in vitro of ＂Huangjinerbai Powder＂ were carried out by using ear edema method of mice and disk diffusion method. [ Result ] The results of anti-inflammatory test showed that the average swelling degree of mice ear in low-dose group was （1.25±0.78） mg, and that in mediumdose group and high-dose group were （0.51 ± 0.38） and （0.42±0.35） mg. There was extremelt significant difference between test group 3 and control group. The results of antibacterial test in vitro showed that the bacteriostatic value of ＂Huangjinerbai Powder＂ on Staphylococcus aureus, Shigella castellani, Pseudomonas aeruginosa, Escherichia coli and Proteus vulgaris were 21.7, 17.4, 14.6, 16.4 and 28.3 mm respectively. [Conclusion] ＂Huangjinerbai Powder＂ had some anti-inflammatory effects, and it was sensitive to S. aureus, S. castellani, P. aeruginosa, E. coli and P. vulgaris and other bacteria. ＂Huangjinerbai Powder＂ had a broad spectrum of antibacterial activity. Compared with gentamicin, ＂Huangjinerbai Powder＂ had better antibacterial effects.