Abstract: This study was conducted to investigate the preservation effect of different extenders on Tibetan Boar semen at 4 ℃ and to provide scientific basis for reasonable and high-efficient utilization of excellent Tibetan Boar breeder semen.Five different extenders commonly used for preservation of Tibetan Boar semen in room temperature were selected.The sperm motility,sperm deformity rate,and the integrity rate of sperm acrosome and plasma membrane preserved by the five extenders at the 5thday of preservation period at 4 ℃ were statistically evaluated and compared.The results revealed that the NO.2 extenders exhibited the best preservation effect with the sperm motility of 0.54 at the 5thday of preservation period,and the sperm motility,sperm deformity rate and the integrity rate of sperm acrosome and plasma membrane preserved with NO.2 extenders were higher than those of the semen preserved with the other four extenders（P〈0.05）.This study preliminary selected the appropriate extenders for Tibetan Boar semen used at 4 ℃ and laid the foundation for the production and application of Tibetan Boar semen in the future.