Abstract: In order to clarify the effect of cutting on biomass and quality of Leymus chinensis meadow grassland and to determine the suitable cutting time and reasonable stubble height, taking Leymus chinensis meadow steppe in Horqin grassland as the research region, a randomized block experiment was conducted in 2016 by two factors of cutting time and stubble height with 3 levels of cutting time and 4 levels of stubble height. The results showed that the coverage, density and above-ground biomass of the community and the above-ground biomass of Leymus chinensis cut on August 15 were significantly higher than those cut on August 1 （P〈0.05）; with the postponement of cutting time, the CP content of the community and Leymus chinensis decreased gradually, and the ADF and NDF content of community increased gradually; there was a significant difference between cutting on August 1 and September 1 （P〈0.05）, but there was no significant difference from cutting on August 15 （P〉0.05）; while the contents of ADF and NDF of Leymus chinensis exhibited an increasing trend, there was no significant difference among varied treatments （P〉0.05）; the under-ground biomass of 0-10 cm, 10-20 cm and 20-30 cm soil communities increased gradually, while the rhizome biomass of Leymus chinensis decreased gradually; the above-ground biomass of the community with 2 cm stubble height was significantly higher than that with 8 cm stubble height （P〈0.05）, and there was no significant difference from the 5 cm stubble height（P〉0.05）; there was no significant difference in CP content among the different communities with stubble height of 2 cm, 5 cm and 8 cm （P〉0.05）, respectively. To sum up, the suitable cutting time of Leymus chinensis meadow steppe in Horqin grassland is about 15 August, and the suitable stubble height is 2-5 cm.