Abstract: The indicator strains should grow fast, decompose lactose or grow well in milk, be generally sensitive to commonly used antibiotics, and have sensitive restriction in line with the allowable maximum residue limit （MRL）. With these requirments as the selection principle, the growth curves of nine standard strains in common medium and whey were observed. And the sensitivity of them to 15 kinds of antibiotics was also determined. Two strains of Geobacillus stearothermophilus, one strain of Staphylococcus aureus and one strain of Streptococcus thermophilus were selected as indicator strains for development of kit. Stability of drug sensitivity, optimization of culture conditions and stability of activity （shelf life）are needed further study.