Abstract: Susceptible and resistant populations of Pelodiscus sinensis were obtained by artificially challenge with Aeromonas hydrophllao.They were screened by SRAP with 64 pairs of primers.A total of 1 200 fragments were obtained,six of which were greatly different between the susceptible and resistant populations.Among these six SRAP fragments,two had dominant gene frequency in the resistant population.These markers may be candidates for selection of Aeromonas hydrophllao resistant Pelodiscus sinensis.Therefore,a basis was provided for molecular marker-assisted selection and cloning of anti-disease genes of Pelodiscus sinensis.