Abstract: The diseased meat pigeons from a rare bird breeding farm in Jilin Province were examined by epidemiological and clinical symptoms, pathological changes. Using the pathogen isolation, biochemical culture, animal test and other methods, it was confirmed as colibacillosis. Finally, the drug sensitivity test was made on the isolated bacteria. The results suggested that the pathogenic E. coli was susceptible to kanamycin, amikacin and florfenicol.