60 Kunming mice were randomly divided into 5 test groups （A, B, C, D, E）with different doses of Oxytropis glabra DC （60%, 40% ,20%, 10% ,5% ）and a control group. The toxicity test of O. glabra was carried out for 60 days. The results showed that mice that were fed with the diet with adding 20% O. globra all survived within 60 days. And mice in the group of adding 40% O. glabra began to die after 57 days. And mice in the group of adding 60% O. glabra began to die after 45 days. The weight gain rate of mice obviously decreased with the increasing of O. glabra in the diet. The final weight of mice in A, B, C test groups was extremely significantly different with control group （P〈0.01 ）. And the weight gain of mice in D test group was significantly different with control group （P〈0.05） and that in E group had no significant difference with control group. Compared with control group ,the viscera coefficients （heart, spleen, brain and lung） of mice in different dose groups changed little. The liver coefficient and kidney coefficient increased with the increasing of the dose of O. glabra. The higher the content of O. glabra in the diet was, the more seriously mice＇s liver and kidney were swollen. And the differences of mice＇s liver and kidney between A, B, C groups and control group was extremely significant （P〈0.01）, which O. gla/bra would caused the injury to the liver and kidney when the dose of O. glabra reached 20%.

The pathogenic strains B 1 and B2 were isolated from diseased Carassius auratus. And five strains of Bdellovibrio were isolated from the mud in the culture area of crab in Southern Four Lakes and Bdellovibrio BD04 was selected to study its biological characteristics preliminarily. The results showed that Bdellovibrio BD04 could not only lyse fish-pathogenic bacteria, but it could also lyse E. coli C600 and Staphylococcus aureus and other bacteria. And the optimum temperature was 30 ℃ and the optimum pH was 8.2. The research results demonstrated that BdeUovibrio as a kind of biological purification had greater developmental potential and application values.

The environmental adaptability of 50 embryo progenies of Dorper sheep in Baotou region of Inner Mongolia which were introduced from Australia were observed, and their growth and development status were also determined. Results showed that some indexes like rectal temperature, heartbeat, respiration, unhairing and ingestion, etc. of lamb, young and adult Dorper sheep had no obvious significance with them in origin place, furthermore some indexes like body weight, withers height, body length and chest width, etc. of them also met the characteristic requirements of Dorper sheep varieties. It is indicated that Dorper sheep show a good adaptability in Baotou region of Inner Mongolia. It provides relative theoretical basis for the feasibility of introducing Dorper sheep varieties and also has practical significance in guiding the crossbreeding and improvement of meat sheep breeds.

In order to identify the chromosomes of Zingibe zerumbet and preliminarily reveal its genome structure, CPD （combined PI and DAPI）staining and fluorescence in situ hybridization （FISH） with 45S rDNA probe were applied to analyze the mitotic metaphase chromosomes. Results showed that two pairs of 45S rDNA sites were respectively detected on the short arms of the chromosome 5 and 10, corresponding to the prominent red CPD bands. The molecular cytogenetic karyotypes of Zingibe zerumbet was constructed based on the data of rDNA and chromosome measurements.

Banana stalk residue silage was added in the growing process of fattening pig. The feeding experiment showed that fattening pigs grew well and had no abnormal reaction. The weight gain of fattening pigs in the test group with adding banana stalk residue silage was significantly lower than that in control group, but the feed-meat rate in the test was 4.73% lower than that in control group. The consumption of mixed feed was effectively decreased. Based on the preliminary calculation, suppose that 0.50 kg banana stalk residue silage was added in the feed of fattening pig, the cost of concentrated feed could save 0.45 yuan per each kilogram of weight gain.

Starling from the current status of pig production in Hengyang City of Hunan Province, the amount of live pigs, the number of slaughtered pigs, pork production and pork prices in Hengyang City during the past ten years were analyzed in detail. It was found that the overall level of pig production in Hengyang was higher and the operation was well conducted. The pig prices fluctuated periodically and a period is about 3-4 years. Some suggestions were put forward, including continuing to consolidate the production status of live pigs in Hengyang and make it stable, establishing a perfect system of veterinary service system and perfecting the market system of products and speeding up the industrialization development.

[Objective] The aim was to study effects of traditional Chinese medicine additive（TCMA）on the growth performance of Hainan Wenchang chicken and to evaluate its safety to mouse. [Method] 180 female Wenchang chicken were randomly assigned to 5 groups with 3 replications each,each replication contain 12 chickens. Diets containing 0 and 600 mg/kg aureomycin as well as 0. 1%, 0.2% and 0.3% TCMA were fed to group 1 ,group 2,goup 3 ,group 4 and group 5 ,respectively. The test for growth lasted 6 weeks,among them body weight was measured per 7 days. Acute toxicity test and accumulation toxicity test were used to evaluate the safety of TCMA. [Result] Ultimate body weight in 0.2% TCMA was improved significantly （P〈0.05）. The median lethal dose （LD50）for mouse was more than 10 g/（kg-bw）, and the accumulation coefficient K was more than 5. [Conclusion] TCMA is a safe feed additive with high performance.

It is difficult to cultivate all flora in animal gastrointestinal tract by using traditional methods, so it is difficult to identify the difference of animal gastrointestinal microbes in each stage. Denaturing gradient gel electrophoresis （DGGE） can avoid the disadvantages of traditional methods and can confirm the difference in animal gastrointestinal microbiota system and the diversity of flora. The basic principle, advantages and disadvantages of DGGE and its application in animal gastrointestinal microbiota system were mainly summarized in this paper.

Protein is an essential nutrient for animal. For non-ruminant animals,protein nutrition is virtual amino acid/small peptide nutrition. Because of the special digestive physiological stucture and microbial digestion for ruminant, the protein nutrition is more complicated than non-ruminant animals. The evaluation technology for the nutritive value of protein is the basis and prerequisite for studying the protein nutrition of animals. Aim at the digestive physiological characteristics of ruminant, some valuation technologies for the nutritive value of protein in ruminant like nylon bag technology, microbial labeling technique, isotopic method, purine derivatives and artificial rumen, etc. were reviewed in this paper. This review is helpful to theoretical research and productive practice.

Strengthening the psychological health of college students is an important measure to fully implement the Party＇s education policy and the quality education under new situation. Health psychology is a very important factor in the growth of college students. Several main problems in the psychological health of current college students were analyzed. And some methods for strengthening and improving the psychological health of college students were given.

This paper analyzes the epigenetie inheritance characteristics of DNA methylation, and combined with the regulation pattern of DNA methylation in different species and different developmental stages of embryos, the role of DNA methylation in early development of embryos regulation of gene expression is reviewed from the perspective of early embryonic death, and thus to clarify the mechanism of epigenetic regulation during embryonic development.

With the development of tumor research, it is found that of epigenetic alterations are also closely related with the development and progression of tumors besides gene mutations. The change of tumor epigenetic inheritance are mainly presented as the aberrant methylation of DNA. The aberrant methylation of DNA occurs in almost all kinds of human tumors, including cutaneous malignancies. DNA methylation refers to the process that under the effect of DNA methylase, methyl is transferred onto a special basic group with S- adenosine-L-methionine （SAM）as the methyl donor. The normal function of gene not only depends on a normal structure, but also depends on a normal state of methylation. The aberrant methylation of DNA takes an important part in the occurrence of tumor.

The principles, analysis method of identifying the species and genus of animals by using the physical and chemical examination and its application situations in the practice were introduced. The advantages and disadvantages of identifying the species and genus of animals by using physical and chemical examination were appraised. It was proposed that a lot of experimental works should be done in the species and genus identification of animals by using physical and chemical examination. And the convenience of the identification method, low identification cost, multi-knowledge amalgamation and union with computer technology were the development direction of animal species identification.

This issue mainly deals with the roles of gonadotropin releasing hormone （GnRH）, melatonin and kisspeptin in the control of animal seasonal reproduction, which are closely related to animal seasonal reproduction.

Based on extensive literature search,the ingredients,pharmacology and clinical applications of Hibiscus syriacus L. were reviewed so as to provide references for the further development of Hibiscus syriacus L.

The compound preparation ＂Lianteng＂ was composed of Chinese herbal medicine Forsythia suspensa （Thunb.）Vahl and Caulis sinomenii. The effective components of this compound preparation were extracted by using water decoction and ethanol extraction method. By acute toxicity test, maximum tolerance test and abnormal toxicity test, the safety of the compound preparation ＂Lianteng＂ was evaluated. The anti-inflammatory function of the compound preparation ＂Lianteng＂ was detected by using dimethylbenzene-induced auricle-swollen test of mice. The results showed that the compound preparation ＂Lianteng＂ was nontoxic and the maximum tolerance dose was 18 g/（kg.bw）. In the anti-inflammatory test in vivo, the repression rate of swelling in high dose group [ 10.0 g/（kg.bw） ] and medium dose group [5.0 g/（kg.bw） ] of compound preparation ＂Lianteng＂ was obviously higher than that in positive control group （dexamethasone） . The compound preparation ＂Lianteng＂ was safe and nontoxic and it had good anti-inflammatory effects and developmental potential .

On the basis of extensive literature retrieval,the species,chemical composition,pharmacological effect and clinical applications of Photinia Lindl. were reviewed so as to provide reference for the further development and utilization of Photinia Lindl.

Based on studies on the planting technique of alfalfa in slight saline-alkali land and semi-arid area, main points in the planting technique of alfalfa in slight saline-alkali land and semi-arid area were put forward. The budding percentage, emergence rate, turning green conditions and grass production of Medicago sativa cv. Aohan, Medicago sativa L. cv. Zhongmu No. 1, ＂Algonquin＂ and ＂Golden Queen＂ were determined. The further popularization and application of planting technique of alfalfa could effectively relieve the contradiction between grain and grass as well as has important significance in developing new forage resources.

This paper describes the status and gap of milk production of Jilin Province and proposes the vision to expand the production of alfalfa for dairy development. It is discussed that the advantages of alfalfa development of Jilin Province contain the climate, land and technical support. A large amount of data is used to clarify the feeding value of alfalfa. It is proved that cows fed alfalfa has a high economic efficiency based on the existing research results, so it is worthy to be popularized and applied in production.

Six species of Bromus inermis Leyss. from different sources were introduced. The results showed that the overwintering rate of six species in western region of Heilongjiang Province were more than 93% and the growth period was 102-106 d. These six species were suitable for planting in western region of Heilongjiang Province. Among them, Bromus inermis Leyss. cv. Gongnong, Bromus inerm/s Leyss. cv. Neimeng and Bromuz inermis Leyss. cv. Bolan had better production performances than other species and these three species were worthy to be popularized in western region of Heilongjiang Province.

Aiming at the production process of animal food, the design problems of the key marking technique in traceability system of animal food were discussed. The test results of the reader and electronic tag showed that the communication distance and signal intensity gradually increased with the multiple decrease of data transfer rate. When data transfer rate was 60 kbps or 10 kbps, better communication effect could be achieved. And the signal could be accurately identified in the range of 50 m. The parameters should be chosen in the actual application according to the reading quantity of tags, the communication distance and disturbance conditions.

From the product advantages, development potential ,broad market, additional value of production, geographical advantages and other aspects of cheese, the development advantages of cheese in Xinjiang were elaborated.

With the continuous development of agriculture and rural economy,the macro-environment of agricultural production has undergone profound changes. As the basic organizational form of agricultural development under the new situation ,specialized cooperative economic organizations of farmers plays a positive role in the development of agriculture and rural economy. The development status of specialized cooperative economic organizations of farmers in Baoding City of Hebei Province was mainly studied. The existing problems of the specialized cooperative economic organizations of farmers in Baoding City were analyzed and reasonable countermeasures and suggestions were put forward.

From the national policy ,market demand ,product development and other perspectives ,the development opportunities of special animal farming were analyzed. The important effects on the risk prevention were discussed, from objectively looking upon the enterprises propaganda,fully performing the market research,following the rules of special farming and strengthening the scientific raising management, which had certain reference and guidance significance for the healthy development of this industry.

The aim was to investigate the elinieal significance of blood routine examination in pets. Routine blood eheeks including quantitative measurement results （like total leukocyte count, differential leukocyte count, erythroeyte count and index and platelet count and index） and qualitative test result （like blood smear morphologie examination） are adopted so as to provide a reference for pet clinical diagnosis, observation on the therapeutic effect and prognosis of the disease.

The diseased meat pigeons from a rare bird breeding farm in Jilin Province were examined by epidemiological and clinical symptoms, pathological changes. Using the pathogen isolation, biochemical culture, animal test and other methods, it was confirmed as colibacillosis. Finally, the drug sensitivity test was made on the isolated bacteria. The results suggested that the pathogenic E. coli was susceptible to kanamycin, amikacin and florfenicol.