Animal Husbandry and Feed Science ›› 2021, Vol. 42 ›› Issue (3): 102-106.doi: 10.12160/j.issn.1672-5190.2021.03.019

• Animal Disease Prevention and Control • Previous Articles     Next Articles

Investigation of Bovine Leukemia Virus Infection at a Dairy Farm in Daqing City

HE Si-rui1,2, WU Rui1,2, LIAN Shuai1,2, WANG Jian-fa1,2, ZHANG Di1,2, GAO Li1,2   

  1. 1. College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China;
    2. Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Daqing 163319,China
  • Received:2021-02-26 Online:2021-05-30 Published:2021-06-21

Abstract: [Objective] To understand the infection status of bovine leukemia virus (BLV) at a dairy farm in Daqing City. [Method] Blood samples were collected from 107 randomly selected lactating dairy cows. The serological epidemiology investigation of BLV was carried out by ELISA, and was verified by fluorescent quantitative PCR. [Result] The BLV positive rate of ELISA was 31.78% (34/107), and that of fluorescent quantitative PCR was 37.38% (40/107). The coincidence rate of positive results of the two test methods was 85.00%. The dairy cows with high BLV load accounted for 26.17% of the total, and those with low BLV load accounted for 11.21% of the total. [Conclusion] The BLV infection is prevalent at a certain level at the dairy farm, and there is a higher risk of BLV spread among the dairy cows in this herd.

Key words: dairy cow, bovine leukemia virus, ELISA, fluorescent quantitative PCR, epidemiological investigation

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