北方农业学报 ›› 2020, Vol. 48 ›› Issue (2): 70-75.doi: 10.12190/j.issn.2096-1197.2020.02.11

• 植物保护·农业生态环境 • 上一篇    下一篇

胡萝卜根腐病病原菌的分离鉴定

韩凤英1, 王勇1, 于传宗1, 高婧1, 杨叔青1, 贾瑞芳2, 扈顺1, 朱春侠1   

  1. 1.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;
    2.内蒙古农业大学,内蒙古 呼和浩特 010018
  • 收稿日期:2020-02-12 出版日期:2020-04-20 发布日期:2020-07-17
  • 通讯作者: 王勇(1960—),男,研究员,学士,主要从事蔬菜育种与栽培方面的研究工作。
  • 作者简介:韩凤英(1986—),女,助理研究员,硕士,主要从事蔬菜育种方面的研究工作。
  • 基金资助:
    内蒙古自治区农牧业科学院青年创新基金项目(2016QNJJN04); 内蒙古自治区党委组织部“草原英才”工程第五批产业创新人才团队项目

Isolation and identification of carrot root rot caused by Fusarium solani

HAN Fengying1, WANG Yong1, YU Chuanzong1, GAO Jing1, YANG Shuqing1, JIA Ruifang2, HU Shun1, ZHU Chunxia1   

  1. 1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;
    2. Inner Mongolia Agricultural University,Hohhot 010018,China
  • Received:2020-02-12 Online:2020-04-20 Published:2020-07-17

摘要: 【目的】探明内蒙古赤峰市和呼和浩特市胡萝卜根腐病病原菌的种类,为胡萝卜根腐病防治提供参考。【方法】采用常规组织分离法、形态学观察和分子生物学技术相结合的方法对病原菌进行分离与鉴定。【结果】分离到5个菌株,形态学观察和ITS序列分析显示菌株均为茄病镰刀菌(Fusarium solani)。用胡萝卜肉质根回接病原菌验证,均表现出致病性。【结论】明确了茄病镰刀菌(Fusarium solani)是两个地区胡萝卜根腐病的主要致病菌。

关键词: 胡萝卜, 根腐病, 茄病镰刀菌

Abstract: 【Objective】To identify the pathogen species of carrot root rot in Chifeng and Hohhot, Inner Mongolia, and provide reference for the control of carrot root rot.【Methods】Conventional tissue isolation, morphological observation and molecular techniques were used to isolate and identify pathogen.【Results】Morphological observation and ITS sequence analysis showed that all 5 strains isolated were Fusarium solani, and their pathogenicity verified by inoculated back to carrot.【Conclusion】It was clear that Fusarium solani was the main pathogen that caused root rot of carrot in two regions.

Key words: Carrot, Root rot, Fusarium solani

中图分类号: 

  • S436.31