北方农业学报 ›› 2022, Vol. 50 ›› Issue (4): 60-66.doi: 10.12190/j.issn.2096-1197.2022.04.09

• 种质资源 · 植物保护 • 上一篇    下一篇

胡萝卜根腐病拮抗菌活性及杀菌剂的室内毒力评价

范博文1, 李正男1, 张磊1, 付崇毅2, 张艳萍2, 王永2, 孙平平1   

  1. 1.内蒙古农业大学 园艺与植物保护学院,内蒙古 呼和浩特 010011
    2.内蒙古自治区农牧业科学院 蔬菜花卉研究所,内蒙古 呼和浩特 010031
  • 收稿日期:2022-05-13 出版日期:2022-08-20 发布日期:2022-09-30
  • 通讯作者: 孙平平
  • 作者简介:孙平平(1985—),女,讲师,博士,主要从事园艺作物病原及病害防控的研究工作。
    范博文(1998—),女,硕士研究生,研究方向为果树病害综合防控。
  • 基金资助:
    内蒙古自治区科技重大专项项目(2021SZD0040);内蒙古自治区科技计划项目(2020GG0137);内蒙古自治区高等学校科学研究项目(NJZY21458);内蒙古农业大学高层次人才科研启动项目(NDYB2018-14)

Antagonistic bacteria activity against carrot root rot and indoor toxicity evaluation of fungicides

FAN Bowen1, LI Zhengnan1, ZHANG Lei1, FU Chongyi2, ZHANG Yanping2, WANG Yong2, SUN Pingping1   

  1. 1. College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010011,China
    2. Institute of Vegetables and Flowers,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China
  • Received:2022-05-13 Online:2022-08-20 Published:2022-09-30
  • Contact: SUN Pingping

摘要:

【目的】筛选对胡萝卜根腐病病原菌具有拮抗活性的菌株,评价19种杀菌剂对胡萝卜根腐病的室内毒力。【方法】通过皿内拮抗法结合活体活性测定拮抗菌株对胡萝卜根腐病病原菌的抑制效果,利用菌丝生长速率法测定19种杀菌剂对胡萝卜根腐病病原菌的室内毒力抑制效果。【结果】萎缩芽孢杆菌(Bacillus atrophaeus DM3-18)对胡萝卜根腐病病原菌的活体抑制率达72.17%。19种杀菌剂中,多菌灵对胡萝卜根腐病病原菌的抑制活性最好,EC50值为0.002 8 mg/L;咪鲜胺、戊唑醇、甲基硫菌灵、十三吗啉、苯醚·丙环唑、肟菌·戊唑醇、苯甲·嘧菌酯对病原菌也有较好的抑制效果,EC50值均小于20 mg/L;甲霜·恶霉灵对胡萝卜根腐病抑制效果最差,EC50值为8 407.936 8 mg/L。【结论】萎缩芽孢杆菌为胡萝卜根腐病生物防治的有效拮抗菌株,具有进一步开发和应用的潜力;多菌灵对胡萝卜根腐病病原菌的抑制效果较强,可用于该病害的防治。

关键词: 胡萝卜, 根腐病, 拮抗菌, 生物防治, 杀菌剂, 毒力评价

Abstract:

【Objective】Screen the strains having antagonistic activity against carrot root rot pathogen and assess the indoor toxicity of 19 fungicides to carrot root rot.【Methods】The inhibition effect of the antagonistic strain on the pathogen of carrot root rot was determined by in vitro and in vivo antagonism method. The mycelium growth rate method was used to assess the indoor toxicity inhibitory effects of 19 fungicides on the pathogen.【Results】Bacillus atrophaeus DM3-18 had in vivo inhibitory rate of 72.17% on the carrot root rot pathogen. Out of the 19 fungicides,carbendazim had the best inhibitory activity against carrot root rot pathogen,with an EC50 value of 0.002 8 mg/L;prochloraz,tebuconazole,thiophanate methyl,tridemorph,difenoconazole·propiconazole,trifloxystrobin·tebuconazole,and difenoconazole·azoxystrobin also had good inhibitory effects,with EC50 value less than 20 mg/L;with an EC50 value of 8 407.936 8 mg/L,metalaxyl-hymexazol had the worst inhibitory effect.【Conclusion】Bacillus atrophicus is an antagonistic strain for biological control of carrot root rot that has the potential for further development and application. Carbendazim has inhibitory impact on the carrot root rot pathogen and can be used to control the disease.

Key words: Carrot, Root rot, Antagonistic bacteria, Biological control, Fungicide, Toxicity evaluation

中图分类号: 

  • S436.31