蔗糖诱导拟南芥突变体rhd3合成花青素及基因表达分析

doi:10.12190/j.issn.2096-1197.2020.05.10

北方农业学报 ›› 2020, Vol. 48 ›› Issue (5): 55-61.doi: 10.12190/j.issn.2096-1197.2020.05.10

• 种质资源·分子生物学 • 上一篇下一篇

蔗糖诱导拟南芥突变体rhd3合成花青素及基因表达分析

聂可心, 李微, 李小龙, 郭嫒, 张靠稳, 王静

北方民族大学生物科学与工程学院/国家民委生态系统建模及应用重点实验室,宁夏银川 750021

收稿日期:2020-08-24 出版日期:2020-10-20 发布日期:2020-12-17
通讯作者: 王静（1984—）,女,讲师,博士,主要从事植物逆境生理研究工作。
作者简介:聂可心（1999—）,女,本科在读,专业为生物工程。
基金资助:
国家自然科学基金项目（31600202）; 北方民族大学自治区级大学生创新项目（2019-QJ-040）; 西北旱作玉米化肥农药减施增效技术集成与示范项目（2017YFD0201807）

Sucrose-induced anthocyanin biosynthesis and gene expression of endoplasmic reticulum mutant rhd3 in Arabidopsis thaliana

NIE Kexin, LI Wei, LI Xiaolong, GUO Ai, ZHANG Kaowen, WANG Jing

College of Biological Science and Engineering,North Minzu University/Key Laboratory of Ecosystem Modelling and Application of State Nationalities Affairs Commission,Yinchuan 750021,China

Received:2020-08-24 Online:2020-10-20 Published:2020-12-17

摘要/Abstract

摘要： 【目的】研究拟南芥内质网蛋白基因AtRHD3（root hair defective 3）在花青素合成中的作用。【方法】以蔗糖为诱导因素,比较拟南芥野生型Col-0及突变体rhd3中花青素的积累量,并通过实时荧光定量PCR技术分析花青素合成通路中基因的表达差异。【结果】以5%蔗糖（sucrose,Suc）为诱导因素,随培养基中蔗糖浓度的增加,野生型Col-0及突变体rhd3中花青素含量逐渐增加,且突变体rhd3中花青素积累量显著高于野生型Col-0,表明AtRHD3在蔗糖诱导的花青素合成中起负调控作用。与野生型Col-0相比,在突变体rhd3中结构基因AtDFR、AtANS、AtUF3GT和正调控基因AtPAP1、AtPAP2、AtTT8、AtTTG1表达水平较高,而负调控基因AtMYBL2表达水平较低。【结论】在蔗糖诱导的拟南芥花青素合成中,AtRHD3通过影响花青素合成通路结构基因和调控基因的表达水平负调控花青素的合成。

关键词: 拟南芥, 突变体rhd3, 花青素, 基因表达

Abstract: 【Objective】The research aimed to identity the function of Arabidopsis thaliana protein gene AtRHD3（root hair defective 3）in anthocyanin biosynthesis.【Methods】Compared the anthocyanin content induced by sucrose in wild type Col-0 and mutant rhd3 and relative expression of genes in anthocyanin biosynthesis pathway by real-time fluorescent quantitative PCR.【Results】With the increasing content of 5% sucrose,anthocyanin content in wild type Col-0 and mutant rhd3 were increased,and the anthocyanin accumulation in mutant rhd3.It is significantly higher than the wild type Col-0,implied that AtRHD3 played negative role in anthocyanin biosynthesis induced by 5% sucrose.The expression levels of structural genes and regulatory genes of anthocyanin biosynthesis pathway in wild type Col-0 and mutant rhd3 after control and 5% sucrose treatment were analyzed.The results showed that compared with wild type Col-0,the expression levels of structural genes（AtDFR、AtANS、AtUF3GT）and positive regulatory genes（AtPAP1、AtPAP2、AtTT8、AtTTG1）expressed higher level than those in wild type Col-0,while the negative regulator AtMYBL2 expressed lower level.【Conclusion】The study indicated AtRHD3 negatively regulated anthocyanin biosynthesis by affecting the expression levels of structural genes and regulatory genes of anthocyanin biosynthesis pathway in Arabidopsis thaliana induced by sucrose.

Key words: Arabidopsis thaliana, Mutant rhd3, Anthocyanin, Gene expression

中图分类号:

Q943.2

聂可心, 李微, 李小龙, 郭嫒, 张靠稳, 王静. 蔗糖诱导拟南芥突变体rhd3合成花青素及基因表达分析[J]. 北方农业学报, 2020, 48(5): 55-61.

NIE Kexin, LI Wei, LI Xiaolong, GUO Ai, ZHANG Kaowen, WANG Jing. Sucrose-induced anthocyanin biosynthesis and gene expression of endoplasmic reticulum mutant rhd3 in Arabidopsis thaliana[J]. Journal of Northern Agriculture, 2020, 48(5): 55-61.

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蔗糖诱导拟南芥突变体rhd3合成花青素及基因表达分析

Sucrose-induced anthocyanin biosynthesis and gene expression of endoplasmic reticulum mutant rhd3 in Arabidopsis thaliana

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