蚯蚓肽对拟南芥生长和细胞质Ca2+浓度的影响

doi:10.3969/j.issn.2096-1197.2019.04.04

北方农业学报 ›› 2019, Vol. 47 ›› Issue (4): 22-26.doi: 10.3969/j.issn.2096-1197.2019.04.04

• 种质资源·生理生化·分子生物学 • 上一篇下一篇

蚯蚓肽对拟南芥生长和细胞质Ca²⁺浓度的影响

孟令博, 祁智, 孙永伟

内蒙古大学生命科学学院/省部共建草原家畜生殖调控与繁育国家重点实验室,内蒙古呼和浩特 010022

收稿日期:2019-06-30 出版日期:2019-08-20 发布日期:2019-12-10
通讯作者: 孙永伟（1985—）,男,讲师,博士,主要从事植物分子生物学的研究工作。
作者简介:孟令博（1994—）,男,硕士研究生,研究方向为生物化学与分子生物学。
基金资助:
内蒙古自治区科技计划项目（201802083）

Effect of earthworm peptide on Arabidopsis growth and the concentration of Ca²⁺ in the cytoplasm

MENG Lingbo, QI Zhi, SUN Yongwei

State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock,School of Life Sciences,Inner Mongolia University,Hohhot 010022,China

Received:2019-06-30 Online:2019-08-20 Published:2019-12-10

摘要/Abstract

摘要： 为了探讨蚯蚓肽对拟南芥生长及细胞质Ca²⁺浓度的影响,选取9种蚯蚓肽添加到拟南芥生长培养基中,观察其对拟南芥生长的影响。结果表明：蚯蚓肽Lumbricin-PG-27、AVPF、VQ-5、F-1和LumA5对拟南芥生长具有显著促进作用,其中LumA5的促进作用最强。以9种蚯蚓肽溶液处理稳定表达水母发光蛋白基因的拟南芥幼苗,发现蚯蚓肽LumA5可以使拟南芥细胞质钙离子浓度[Ca²⁺]_cyt升高,添加抑制剂Alloxan、GdCl₃、Neomycin可显著抑制LumA5引起的细胞质钙离子浓度[Ca²⁺]_cyt的升高,这一发现为蚯蚓肽应用于农业生产中提供了科学依据。

关键词: 蚯蚓肽, 拟南芥, 细胞质钙离子浓度

Abstract: To investigate the effect of earthworm peptide on Arabidopsis growth,nine earthworm peptides were tested.The results showed that Lumbricin-PG-27,AVPF,VQ-5,F-1,and especially LumA5 had a significant enhancing effect on Arabidopsis growth.An increased[Ca²⁺]_cyt was found in Arabidopsis seedlings treated with LumA5.The results also showed that the Alloxan,GdCl₃,and Neomycin can inhibit the increase of [Ca²⁺]_cyt treated with LumA5.This study provides scientific evidence for the application of earthworm peptides in agricultural production.

Key words: Earthworm peptide, Arabidopsis, [Ca²⁺]_cyt

中图分类号:

S184

孟令博, 祁智, 孙永伟. 蚯蚓肽对拟南芥生长和细胞质Ca²⁺浓度的影响[J]. 北方农业学报, 2019, 47(4): 22-26.

MENG Lingbo, QI Zhi, SUN Yongwei. Effect of earthworm peptide on Arabidopsis growth and the concentration of Ca²⁺ in the cytoplasm[J]. Journal of Northern Agriculture, 2019, 47(4): 22-26.

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蚯蚓肽对拟南芥生长和细胞质Ca²⁺浓度的影响

Effect of earthworm peptide on Arabidopsis growth and the concentration of Ca²⁺ in the cytoplasm

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