关键词: 小麦, 脱氧雪腐镰刀菌烯醇, 赤霉病病粒率, 容重, 液相色谱串联质谱法

Abstract:

【Objective】To explore the accumulation of deoxynivalenol（DON） in the kernels of different wheat varieties under natural disease conditions and the effect on Fuasrium diseased kernel rate（FDK） and test weight，so as to provide reference for breeding wheat varieties resistant to Fuasrium.【Methods】The content of DON，3A-DON（3-acetyldeoxynivalenol） and 15A-DON（15-acetyldeoxynivalenol） in the kernels of 65 wheat varieties from different sources were tested by liquid chromatography-tandem mass spectrometry（LC-MS/MS） to analyze the difference in Fuasrium diseased kernel rate（FDK） and test weight （TW） under different DON content.【Results】The FDK of the 65 wheat varieties ranged from 0.20% to 7.80%. The content of DON was 0.11 to 4.26 mg/kg. The test weight was 703.00 to 791.70 g/L. With correlation coefficients of 0.514，0.549，0.529 and 0.521，respectively，FDK was significantly positively correlated with the contents of DON，3A-DON，15A-DON and DON-Total（P<0.01），and was significantly negatively correlated with test weight（P<0.05），with correlation coefficients of -0.254. With correlation coefficients of 0.999，0.911 and 0.899，respectively，the kernel contents of DON，3A-DON，15A-DON had significant positive correlation（P<0.01） with DON-Total，and significant negative correlation with test weight（P<0.01），with correlation coefficients of -0.502，-0.504 and -0.481，respectively. In terms of the source of the varieties，the kernel accumulation of DON was lowest in Jiangsu（1.51 mg/kg），followed by Anhui（2.06 mg/kg），Henan（2.75 mg/kg） and Shandong（2.98 mg/kg）. The content of 3A-DON was lowest in Jiangsu（0.047 mg/kg），followed by Anhui（0.075 mg/kg），Shandong（0.111 mg/kg） and Henan（0.113 mg/kg）. The content of 15A-DON was lowest in Jiangsu（0.059 mg/kg），followed by Anhui（0.090 mg/kg），Shandong（0.132 mg/kg）and Henan（0.141 mg/kg）. The content of DON-Total was lowest in Jiangsu（1.61 mg/kg），followed by Anhui（2.23 mg/kg），Henan（3.00 mg/kg）and Shandong（3.23 mg/kg）. The number and proportion of varieties with DON no more than 2 mg/kg in wheat kernels were as follows：Anhui（18，64.29%）>Jiangsu（13，61.90%）>Shandong（2，22.22%）>Henan（1，14.29%）. The average test weight was highest in Jiangsu（758.57 g/L），followed by Anhui（751.90 g/L），Henan（739.71 g/L） and Shandong（735.27 g/L）. In terms of the characteristics of different varieties，eight wheat varieties with DON no more than 2 mg/kg and test weight no less than 770.00 g/L were selected. Among these，there were seven semi winter varieties including Huaimai 40，Huaimai 44，Nongmai 152，Quanmai 725，Womai No. 9，Xunong 029 and Huaimai 45，one spring variety，Guohong No. 6.【Conclusion】The kernel FDK，DON contents and test weight of the 65 wheat varieties were quite different. With the increase of FDK，the kernel accumulation of 3A-DON，15A-DON and DON-Total increased gradually，and the average test weight decreased. Huaimai 40，Huaimai 44，Nongmai 152，Quanmai 725，Womai No.9，Xunong 029，Huaimai 45，and Guohong No. 6 had better resistance to Fuasrium disease.

Key words: Wheat, Deoxynivalenol（DON）, Fuasrium diseased kernel rate（FDK）, Test weight, Liquid chromatography-tandem mass spectrometry（LC-MS/MS）