草原黑蘑菌丝体生长培养条件筛选

doi:10.12190/j.issn.2096-1197.2025.02.02

北方农业学报 ›› 2025, Vol. 53 ›› Issue (2): 10-12.doi: 10.12190/j.issn.2096-1197.2025.02.02

• 分子生物学·生理生化 • 上一篇下一篇

草原黑蘑菌丝体生长培养条件筛选

王海燕^1,2, 于传宗^1,2, 吴媛³, 李亚娇^1,2, 李敏宇^1,2, 狄洁增^1,2, 丁志颖^1,2, 孙国琴^1,2

1.内蒙古自治区农牧业科学院蔬菜花卉研究所,内蒙古呼和浩特 010031;
2.食用菌内蒙古自治区工程研究中心,内蒙古呼和浩特 010031;
3.固阳县农业技术推广中心,内蒙古固阳 014200

收稿日期:2024-10-28 出版日期:2025-04-20 发布日期:2025-09-08
通讯作者: 孙国琴（1964—）,女,研究员,硕士,主要从事食用菌资源开发利用方面的研究工作。
作者简介:王海燕（1984—）,女,副研究员,硕士,主要从事食用菌种质资源创新与新品种选育方面的研究工作。

Screening of mycelial culture conditions for the growth of Agaricus arvensis

WANG Haiyan^1,2, YU Chuanzong^1,2, WU Yuan³, LI Yajiao^1,2, LI Minyu^1,2, DI Jiezeng^1,2, DING Zhiying^1,2, SUN Guoqin^1,2

1. Institute of Vegetables and Flowers,Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Hohhot 010031,China;
2. Engineering Research Center of Edible Mushroom in Inner Mongolia Autonomous Region,Hohhot 010031,China;
3. Guyang County Agricultural Technology Extension Center,Guyang 014200,China

Received:2024-10-28 Online:2025-04-20 Published:2025-09-08

摘要/Abstract

摘要： 【目的】筛选适宜草原黑蘑菌丝体生长的培养基配方,为培育高质量菌种、实现规模化生产提供依据。【方法】以草原黑蘑菌株a72、a99、a100为试验材料,研究不同碳源（葡萄糖、果糖、蔗糖、麦芽糖、乳糖、可溶性淀粉、玉米淀粉、纤维二糖、甘露糖）、氮源（蛋白胨、酵母粉、酵母膏、牛肉膏、硫酸铵、磷酸氢二铵、牛肉粉、干酪素、谷氨酰胺）、pH值（4.5、5.0、5.5、6.0、6.5、7.0、7.5、8.0、8.5）对菌丝体生长速度、菌落长势和生长指数的影响,并通过3因素3水平正交试验筛选适宜其生长的培养基配方。【结果】适宜a72、a99、a100菌株菌丝体生长的碳源均为蔗糖,其生长速度分别为2.84、2.79、3.41 mm/d,生长指数分别为11.360、12.560、15.350。适宜a72菌株菌丝体生长的氮源为谷氨酰胺,其生长速度和生长指数为3.43 mm/d和17.150;适宜a99、a100菌株菌丝体生长的氮源为磷酸氢二铵,其生长速度分别为4.19、3.85 mm/d,生长指数分别为18.855、17.325。适宜3个菌株菌丝体生长的pH值均为6.5。3因素3水平正交试验结果表明,适宜a72菌株菌丝体生长的培养基配方为25 g/L蔗糖、2.0 g/L谷氨酰胺、pH值6.0,适宜a99菌株菌丝体生长的培养基配方为25 g/L蔗糖、2.0 g/L磷酸氢二铵、pH值6.0,适宜a100菌株菌丝体生长的培养基配方为25 g/L蔗糖、2.5 g/L磷酸氢二铵、pH值6.0。【结论】草原黑蘑a72、a99、a100菌丝体更适宜在中性偏酸性的环境条件下生长,3因素3水平正交试验筛选出了适宜3个菌株菌丝体生长的培养基配方。

关键词: 草原黑蘑, 菌丝体, 碳源, 氮源, pH值, 生长速度, 生长指数

Abstract: 【Objective】To screen the optimal culture medium formulation for the mycelial growth of Agaricus arvensis,providing a basis for cultivating high-quality strains and achieving large-scale production.【Methods】Using Agaricus arvensis strains a72,a99,and a100 as test materials,the effects of different carbon sources（glucose,fructose,sucrose,maltose,lactose,soluble starch,corn starch,cellobiose,mannose）,nitrogen sources（peptone,yeast powder,yeast extract,beef extract,ammonium sulfate,diammonium hydrogen phosphate,beef powder,casein,glutamine）,and pH values（4.5,5.0,5.5,6.0,6.5,7.0,7.5,8.0,8.5） on mycelial growth rate,colony vigor,and growth index were investigated. An orthogonal experiment with three-factor,three-level was conducted to determine the optimal culture medium formulation for mycelial growth.【Results】Sucrose was identified as the optimal carbon source for the mycelial growth of strains a72,a99,and a100,with growth rates of 2.84,2.79,and 3.41 mm/d,and growth indices of 11.360,12.560,and 15.350,respectively. The optimal nitrogen source for strain a72 was glutamine,with a growth rate of 3.43 mm/d and a growth index of 17.150,while diammonium hydrogen phosphate was optimal for strains a99 and a100,with growth rates of 4.19 and 3.85 mm/d,and growth indices of 18.855 and 17.325,respectively. The optimal pH value for mycelial growth of all three strains was 6.5. The three-factor,three-level orthogonal experiment revealed that the optimal culture medium formulations were：for strain a72,25 g/L sucrose,2.0 g/L glutamine,pH 6.0;for strain a99,25 g/L sucrose,2.0 g/L diammonium hydrogen phosphate,pH 6.0;and for strain a100,25 g/L sucrose,2.5 g/L diammonium hydrogen phosphate,pH 6.0.【Conclusion】The mycelial of Agaricus arvensis strains a72,a99,and a100 are more suitable for growth under slightly acidic to neutral environmental conditions. The three-factor,three-level orthogonal experiment successfully identified optimal culture medium formulations for the mycelial growth of these strains.

Key words: Agaricus arvensis, Mycelium, Carbon source, Nitrogen source, pH value, Growth rate, Growth index

中图分类号:

S646

王海燕, 于传宗, 吴媛, 李亚娇, 李敏宇, 狄洁增, 丁志颖, 孙国琴. 草原黑蘑菌丝体生长培养条件筛选[J]. 北方农业学报, 2025, 53(2): 10-12.

WANG Haiyan, YU Chuanzong, WU Yuan, LI Yajiao, LI Minyu, DI Jiezeng, DING Zhiying, SUN Guoqin. Screening of mycelial culture conditions for the growth of Agaricus arvensis[J]. Journal of Northern Agriculture, 2025, 53(2): 10-12.

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草原黑蘑菌丝体生长培养条件筛选

Screening of mycelial culture conditions for the growth of Agaricus arvensis

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