北方农业学报 ›› 2018, Vol. 46 ›› Issue (5): 39-43.doi: 10.3969/j.issn.2096-1197.2018.05.06

• 著者文摘 • 上一篇    下一篇

转录组测序及其在甜菜功能基因挖掘中的应用

张自强;白晨;张惠忠;李晓东;付增娟;赵尚敏;鄂圆圆;张辉;王良;张必周   

  1. 内蒙古农牧业科学院特色作物研究所,内蒙古呼和浩特010031
  • 收稿日期:2018-07-20 出版日期:2018-08-19 发布日期:2019-08-19
  • 作者简介:张自强(1984-),男,助理研究员,博士,主要从事甜菜分子育种的研究工作。
  • 基金资助:
    内蒙古自治区自然科学基金项目[2016Bs(LH)0305];农业部糖料产业技术体系项目(CARS-170104)

Transcriptome sequencing and its application in sugar beet functional gene resource discovery

ZHANG Ziqiang[1];BAI Chen[1];ZHANG Huizhong[1];LI Xixodong[1];FU Zengjuan[1];ZHAO Shangmin E Yuanyuan[1];ZHANG Hui[1];WANG Liang[1];ZHANG Bizhou[1]   

  1. Special Crop Institute,Inner Monlolia Academy of Agricultural and Animal HusbandPy Sciences,Hohhot 010031,China
  • Received:2018-07-20 Online:2018-08-19 Published:2019-08-19

摘要: 由于转录组测序技术具有检测费用低、检测通量高、不受遗传背景影响等优势,使其成为当前挖掘基因的主要手段之一。文章首先个绍了转录组测序技术的基本情况,接着阐述了该技术在甜菜功能基因研究方面的进展,文章最后展望转录组测序技术在甜菜基因挖掘中的应用前景。通过转录组测序可以构建甜菜丰富的基因表达数据库,尤其是挖掘与甜菜产量、台糖量调控、抗逆性相关的基因资源,为后续基因克隆、分子标记辅助育种以及转基因育种等提供理论支持与基因资源。

关键词: 转录组测序;甜菜;功能基因挖掘

Abstract: Transcriptome sequencing technology had many advantages, such as low cost, high detection flux, and no influence on genetic background. In this paper, the status of the transcriptional sequencing technology was summarized, and then the research progress of its use in the functional genomics of beet was expounded. Finally, the prospect of its application in the sugar beet gene excavation was elaborated. The rich gene database of sugar beet can be constructed by the sequencing of transcriptional groups, especially for the gene resources related to beet yield and the regulation of sugar content and resistance. These constructs provide theoretical support and genetic resources for subsequent gene cloning, molecular marker-assisted breeding and transgenic breeding.

Key words: RNA-Seq;Sugarbeet;Functional gene excavation

中图分类号: 

  • S566.3