VNTR分子标记在甜菜细胞质类型鉴定的应用研究

doi:10.12190/j.issn.2096-1197.2022.01.01

北方农业学报 ›› 2022, Vol. 50 ›› Issue (1): 1-10.doi: 10.12190/j.issn.2096-1197.2022.01.01

• 分子生物学・种质资源 • 下一篇

VNTR分子标记在甜菜细胞质类型鉴定的应用研究

张辉, 王良, 付增娟, 李晓东, 赵尚敏, 鄂圆圆, 郑文哲, 张自强, 张必周, 张惠忠

内蒙古自治区农牧业科学院,内蒙古呼和浩特 010031

收稿日期:2021-12-21 出版日期:2022-02-20 发布日期:2022-04-11
通讯作者: 张惠忠（1966—）,男,研究员,学士,主要从事甜菜遗传育种的研究工作。
作者简介:张辉（1985—）,男,副研究员,博士,主要从事甜菜遗传育种与生物技术的研究工作。
基金资助:
内蒙古自治区科技计划项目（2021GG0026）; 内蒙古自治区重大专项项目（ZDZX201815）; 现代农业产业技术体系建设专项资金项目

Research of VNTR molecular markers application in the cytoplasm type identification of sugarbeet

ZHANG Hui，WANG Liang，FU Zengjuan，LI Xiaodong，ZHAO Shangmin，E Yuanyuan，ZHENG Wenzhe，ZHANG Ziqiang，ZHANG Bizhou，ZHANG Huizhong

Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China

Received:2021-12-21 Online:2022-02-20 Published:2022-04-11

摘要/Abstract

摘要： 【目的】对应用中的甜菜成对保持系和不育系材料进行细胞质类型鉴定,为甜菜保持系和不育系的选育提供理论依据和技术指导。【方法】结合田间育性性状调查,利用开发出的VNTR特异引物TR1、TR2、TR3和TR4对40份甜菜试验材料进行分子鉴定,根据特异性条带区分细胞质类型。【结果】21份拟入选不育类型的材料中检测出符合Owen型的材料有N9849-17-1、N9865-103-C1、N9857-5-1-TH1-400、MS321-C27-1-80、MS331-N70、MS343-80、MS117-3-6-4-2、MS301-500、MS351-80、MS327-70-80、MS333-70-80、MS335-N70、2068B-2、MS151-1-16-301-400、MS317-1-8-301、MS313-506-600、MS320-7-605-1-84,其中MS329-N70、MS323-503-600分别为N1和N2型细胞质;19份拟入选可育型材料中检测出符合N1型细胞质的材料有960767-201TH-1、960764-1-11-1-1-400、OT322-C7-70-80、OT332-N70、OT352-80、OT328-70-80、BS301-13-9、OT352-80-1、OT342-80、OT152-1-6-301-400,符合N2型细胞质的材料有OT302-500,其中960766-1077-c1、OT344-80、OT118-4-5-4-4、OT324-501-600为Owen型细胞质类型;分子鉴定结果不纯的材料有6份（MS351-80-1、MS341-80、OT152-1-1-C301、OT330-N70、OT334-70-80、OT336-N70）。田间育性调查对应分子鉴定结果表明,960766-1077-c1、OT344-80、OT118-4-5-4-4和OT324-501-600均为半可育型,细胞质类型属于Owen型细胞质;MS329-N70和MS323-503-600属于半不育二型,细胞质类型不属于Owen型细胞质。【结论】已开发的VNTR特异引物的应用可以鉴定甜菜保持系和不育系的细胞质类型,且与田间育性性状调查结果基本匹配。

关键词: 甜菜, VNTR, 细胞质类型, 育性鉴定

Abstract: 【Objective】Sugarbeet paired maintainer lines and male sterile lines′ cytoplasmic types were determined in order to provide theoretical basis and technical advice for breeding sugarbeet maintainer lines and male sterile lines.【Methods】40 sugarbeet test materials were identified using the generated VNTR specific primers TR1, TR2, TR3,and TR4, and the cytoplasmic types were separated according to the particular bands, which was combined with the research of fertility features in the field.【Results】Among the 21 materials to be selected for sterility type,the materials conforming to be Owen type were N9849-17-1,N9865-103-C1, N9857-5-1-TH1-400, MS321-C27-1-80, MS331-N70, MS343-80, MS117-3-6-4-2, MS301-500, MS351-80, MS327-70-80, MS333-70-80, MS335-N70, 2068B-2, MS151-1-16-301-400, MS317-1-8-301, MS313-506-600 and MS320-7-605-1-84. MS329-N70 was cytoplasm type N1 and MS323-503-600 was N2. Among the 19 fertile materials to be selected, 960767-201TH-1, 960764-1-11-1-1-400, OT322-C7-70-80, OT332-N70, OT352-80, OT328-70-80, BS301-13-9, OT352-80-1, OT342-80 and OT152-1-6-301-400 were found to be in line with the cytoplasm type N1, and OT302-500 was found to be in line with the cytoplasm type N2. 960766-1077-c1, OT344-80, OT118-4-5-4-4 and OT324-501-600 were Owen type. Six materials with inconsistent molecular identification results were MS351-80-1, MS341-80, OT152-1-1-1-C301, OT330-N70, OT334-70-80, and OT336-N70. The results of field investigation revealed that 960766-1077-c1, OT344-80, OT118-4-5-4-4 and OT324-501-600 were semi-fertile with Owen cytoplasmic types. MS329-N70 and MS323-503-600 were semi-sterile type Ⅱ and were not Owen cytoplasmic type.【Conclusion】The VNTR specific primers developed could be utilized to distinguish the cytoplasmic types of sugarbeet maintainer lines and male sterile lines. The results were consistent with the results of the field fertility feature identification.

Key words: Sugarbeet, VNTR, Cytoplasmic type, Fertility identification

中图分类号:

S566.3

张辉, 王良, 付增娟, 李晓东, 赵尚敏, 鄂圆圆, 郑文哲, 张自强, 张必周, 张惠忠. VNTR分子标记在甜菜细胞质类型鉴定的应用研究[J]. 北方农业学报, 2022, 50(1): 1-10.

ZHANG Hui，WANG Liang，FU Zengjuan，LI Xiaodong，ZHAO Shangmin，E Yuanyuan，ZHENG Wenzhe，ZHANG Ziqiang，ZHANG Bizhou，ZHANG Huizhong. Research of VNTR molecular markers application in the cytoplasm type identification of sugarbeet[J]. Journal of Northern Agriculture, 2022, 50(1): 1-10.

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VNTR分子标记在甜菜细胞质类型鉴定的应用研究

Research of VNTR molecular markers application in the cytoplasm type identification of sugarbeet

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