Abstract: In order to solve the problem that the low nitrogen fertilizer efficiency of high yield corn limit coordination of high yield and efficient, we took experiment to made clear the nitrogen use efficiency of high-yielding spring maize.The materials are different genotype maize varieties.We set different N applications （0, 30, 450, 600 kg/hm2） and comparison analysised the differences of the nitrogen absorption and utilization, transfer characteristics and nitrogen use efficiency of high yield spring corn.The results showed that with increaing nitrogen rate, the lower yield increased, and the nitrogen harvest index decreased when N application was more than 300 kg/hm2. The nitrogen absorption increase first then decreased.N physiological use efficiency declined slightly. The nitrogen uptake efficiency significantly correlated to utilization efficiency. It was the reason to N fertilizer efficiency dropped sharply that the nitrogen uptake efficiency significantly decreased.