北方农业学报 ›› 2016, Vol. 44 ›› Issue (3): 33-33.

• 研究报告 • 上一篇    下一篇

草莓茎尖组织培养防褐化技术优化研究

付崇毅[1];姜伟[1];郝利清[2];王建国[1];杜金伟[1]   

  1. [1]内蒙古农牧业科学院,内蒙古呼和浩特010031 [2]内蒙古商都县林业工作站,内蒙古商都013450
  • 出版日期:2016-06-20 发布日期:2016-06-20
  • 通讯作者: 付崇毅
  • 作者简介:付崇毅(1986-),男,助理研究员,博士,研究方向为设施园艺及抗逆生理。
  • 基金资助:
    内蒙古农牧业创新基金(2013CXJJN09);内蒙古农牧业科学院青年基金(2015QNJJN01):

Study on technical optimization of preventing browning for strawberry in stem-tip culture

FU Chongyi, JIANG Wei, HAO Liqing, WANG Jianguo, DU Jinwei (1.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China; 2.Forestry Work Station of Shangdu County,Shangdu 013450,China)   

  • Online:2016-06-20 Published:2016-06-20

摘要: 以草莓茎尖为试验材料,研究了不同维生素(VC)溶液浓度及处理时间对草莓茎尖抗褐化效果的影响,培养基中添加抗氧化剂或吸附剂对草莓茎尖抗褐化效果的影响。结果表明:采用VC溶液对草莓顶芽进行浸泡预处理,最适宜浓度为300 mg/L,最适宜浸泡时间为12 min。VC溶液浸泡预处理后在培养基中添加1.5 g/L PVP对草莓茎尖褐化的发生抑制效果最好,茎尖愈伤组织萌发率达到100%。

Abstract: In order to study- the effect of different immersion concentration and time of Vc, adding antioxidant and adsorbentin medium on preventing browning for strawberry in stem-tip culture, the strawbmfy stem tips were selected. The resuhsshowed that the browning can be controlled by soaking the strawbmfy terminal bud in 300 mg/L Vc solution for 12 rain. Atthe same time, the inhibition effect of PVP added in the medium with 1.5 g/L on the browning of strawberry stem tip was thebest, and the germination rate of the callus was 100% for strawberry terminal bud.

中图分类号: 

  • S668.4