大兴安岭森林表层土壤真菌多样性的分析

北方农业学报 ›› 2016, Vol. 44 ›› Issue (3): 5-5.

大兴安岭森林表层土壤真菌多样性的分析

冀锦华[1];王小兵[2];孟建宇[1];缪恺[3]

[1]内蒙古农业大学生命科学学院应用与环境微生物研究所,内蒙古呼和浩特010018 [2]内蒙古农牧业科学院作物育种与栽培研究所,内蒙古呼和浩特010031 [3]锡林郭勒盟创绿环境监理公司,内蒙古锡林浩特026000

出版日期:2016-06-20 发布日期:2016-06-20
通讯作者: 冀锦华
作者简介:冀锦华（1990-），男，硕士研究生，研究方向为纤维素的微生物降解。通讯作者：孟建宇（1976-），男，副教授，硕士，主要研究方向为微生物降解与可再生能源。
基金资助:
国家自然基金项目（31460002）

Analysis of fungal diversity in the surface soil of Great Khingan forest

JI Jinhua, WANG Xiaobing, MENG Jianyu, MIAO Kai （ 1.Institution for Applied and Environmental Microbiology of Life Sciences College, Inner Mongolia Agricultural University, Hohhot 010018, China ;2.Institution for Crop Breeding and Cultivation, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;3. Xilingol League Chuanglv Environmental Supervision Company, Xilinhot 026000,China）

Online:2016-06-20 Published:2016-06-20

摘要/Abstract

摘要： 为了系统地揭示大兴安岭森林土壤真菌多样性,该研究采集了位于大兴安岭森林生态系统定位站附近森林表层土壤,基于ITS序列利用Miseq高通量测序技术分析真菌的多样性。结果表明：测序得到3 260条可用ITS序列;在0.03差异水平下,它们共分为51个OTU,Alpha多样性指数Shannon为0.818 9,覆盖度（Coverage）为85.6%,指数chao和ace估算的OTU分别为85和155;这些序列主要来自担子菌门（Basidiomycota）、子囊菌门（Ascomycota）、接合菌门（Zygomycota）及一小部分未分类类群和未分类的真菌菌群,其中担子菌门为绝对优势类群,比例高达97.91%。

Abstract: To assess the diversity of fungi in soil of Great Khingan forest, the forest surface soil around the Ecosystem Station ofGreat Khingan was collected. Then based on ITS gene,the Miseq high-throughput sequencing technique was applied to discoverthe diversity of fungi in the soil. The resuhs showed that obtained 3 260 ITS sequences could be usable. At the divergence levelof 0.03, these sequences were divided into 51 OTU. The alpha diversity indexes namely Shannon,coverage, Chao value and acevalue were 0.8189,85.60% ,85 and 155, respectively. The ITS sequences were originated from Basidiomycota, Ascomycota,Zygomycota and some unknown tungal taxa, of which the predominated was Basidiomycota with the highest ratio of 97.91%.

中图分类号:

S714.3

冀锦华王小兵孟建宇缪恺. 大兴安岭森林表层土壤真菌多样性的分析[J]. 北方农业学报, 2016, 44(3): 5-5.