Abstract: Using 4 domestic maize inbred lines（Zheng 58,Chang7-2,Four-144 and Four-287） as testing materials, the combining ability and check heterosis of 16 American maize inbred lines were evaluated by NCII genetic mating design.To screen the American maize inbred lines with good combining ability and hybrid combinations with excellent characteristics as well as to provide theory basis for their reasonable utilization, the utilization potential of the 16 American maize inbred lines were analyzed. Among the testing American inbred lines, the LH205, 2 FACC, LH196 and LH190 showed excellent product characteristics GCA effect; the PHV37, PHW03, 2 FACC, LH163, LH206, LH220 Ht,LH160, LH162 showed excellent machine-harvesting characteristics GCA effect. In hybrid combinations, the LH160 ×Zheng 58, 2 FACC ×Chang 7-2, PHW43 ×Four-144, LH195 ×Four-287, LH220 Ht ×Four-287, LH190 ×Four-287,LH202 ×Four-287 showed excellent yield characteristics SCA effect; the 2 FACC ×Chang 7-2, LH195 ×Four-287,2 FACC ×Four-144, LH162 ×Chang 7-2,LH205 ×Chang 7-2,LH190 ×Four 287 showed excellent output TCA effect; the2 FACC ×Chang 7-2, LH195 ×Four-287, and 2 FACC ×Four-144 showed excellent advantages comparing to the check,which can be further verified in following test.