水稻稻瘟病抗病基因挖掘及育种研究进展

doi:10.12190/j.issn.2096-1197.2021.02.15

摘要/Abstract

摘要： 水稻稻瘟病的发生会对水稻产量造成巨大损失,严重限制水稻的生产。利用宿主自身抗病基因,培育稳定、有效、广谱、持久的抗病品种是控制稻瘟病最安全有效和经济的方法。随着分子生物学技术的飞速发展,更多的稻瘟病抗病基因和QTLs被挖掘用于创建稳定、广谱、持久的抗病材料。文章综述了已克隆抗病基因和QTLs的结构、功能和抗病机制以及这些抗病基因结合的分子标记选择辅助育种技术、聚合抗病基因和基因编辑技术在抗病育种中的应用实例,并对抗稻瘟病育种的前景进行了展望。

关键词: 稻瘟病, 抗病基因, 分子标记, 基因聚合, 基因编辑, 育种

Abstract: Rice blast caused great loss to rice yield and severely restricted rice production. It was the safest,most effective and economical method to breed stable,effective,broad-spectrum and long-lasting varieties of rice blast resistance by using the host′s own disease-resistant genes. With the rapid development of molecular biotechnology,more rice blast resistance genes and QTLs were exploited to create stable,broad-spectrum and long-lasting disease-resistant materials. In this paper,the structure,function and disease-resistance mechanism of cloned disease-resistant genes and QTLs were reviewed,and the application of these disease-resistant genes combined with molecular marker selective breeding,polymerized disease-resistant genes and gene editing techniques in disease-resistant breeding was summarized,and the prospect of rice blast resistance breeding was also discussed.

Key words: Blast, Disease-resistant genes, Molecular markers, Gene aggregation, Gene editing, Breeding

中图分类号:

S511

燕孟娇, 贾晓清, 郝丽芬, 宋培玲, 皇甫海燕, 郭晨, 皇甫九茹, 杨永青, 史志丹, 李子钦. 水稻稻瘟病抗病基因挖掘及育种研究进展[J]. 北方农业学报, 2021, 49(2): 94-103.

YAN Mengjiao, JIA Xiaoqing, HAO Lifen, SONG Peiling, HUANGFU Haiyan, GUO Chen, HUANGFU Jiuru, YANG Yongqing, SHI Zhidan, LI Ziqin. Research progress on rice blast resistance gene exploiting and breeding[J]. Journal of Northern Agriculture, 2021, 49(2): 94-103.

参考文献

[1] ASHKANI S,RAFII M Y,SHABANIMOFRAD M,et al.Molecular breeding strategy and challenges towards improvement of blast disease resistance in rice crop[J].Front Plant Science,2015,6:886.
[2] SHUICHI F,NORIKUNI S,HIRONORI K,et al.Loss of function of a proline-containing protein confers durable disease resistance in rice[J].Science,2009,325(5943):998-1001.
[3] XIAO N,WU Y,PAN C,et al.Improving of rice blast resistances in japonica by pyramiding major R genes[J].Front Plant Science,2016,7:1918.
[4] NELSON R,WIESNER-HANKS T,WISSER R,et al.Navigating complexity to breed disease-resistant crops[J].Nat Rev Genet,2018,19(1):21-33.
[5] CHAIPANYA C,TELEBANCO-YANORIA M J,QUIME B,et al.Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast[J].Rice,2017,10(1):18.
[6] FANG N,WEI X,SHEN L,et al.Fine mapping of a panicle blast resistance gene Pb-bd1 in Japonica landrace Bodao and its application in rice breeding[J].Rice,2019,12(1):18.
[7] SU J,WANG W,HAN J,et al.Functional divergence of duplicated genes results in a novel blast resistance gene Pi50 at the Pi2/9 locus[J].Theoretical & Applied Genetics,2015,128(11):1-13.
[8] VASUDEVAN K,GRUISSEM W,BHULLAR N K.Identification of novel alleles of the rice blast resistance gene Pi54[J].Scientific Reports,2015,5:15678.
[9] ZHENG W,WANG Y,WANG L,et al.Genetic mapping and molecular marker development for Pi65(t),a novel broad-spectrum resistance gene to rice blast using next-generation sequencing[J].Theoretical and Applied Genetics,2016,129(5):1035-1044.
[10] SHINADA H,YAMAMOTO T,SATO H,et al.Quantitative trait loci for rice blast resistance detected in a local rice breeding population by genome-wide association mapping[J].Breeding Science,2015,65(5):388-395.
[11] LIU W,LIU J,TRIPLETT L,et al.Novel insights into rice innate immunity against bacterial and fungal pathogens[J].Annual Review of Phytopathology,2013,52(1):213-241.
[12] WANG B H,EBBOLE D J,WANG Z H.The arms race between Magnaporthe oryzae and rice:Diversity and interaction of Avr and R genes[J].Journal of Integrative Agriculture,2017,16(12):2746-2760.
[13] LI W,ZHU Z,CHERN M,et al.A natural allele of a transcription factor in rice confers broad-spectrum blast resistance[J].Cell,2017,170(1):114-126.
[14] WANG J,LIU X,ZHANG A,et al.A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice[J].Cell Research,2019,29(10):820-831.
[15] DENG Y,ZHAI K,XIE Z,et al.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J].Science,2017,355(6328):962.
[16] JOSHI S,DHATWALIA S,KAACHRA A,et al.Genetic and physical mapping of a new rice blast resistance specificity Pi-67 from a broad spectrum resistant genotype Tetep[J].Euphytica,2019,215(1):9.
[17] CHEN X,SHANG J,CHEN D,et al.A B-lectin receptor kinase gene conferring rice blast resistance[J].Plant Journal,2006,46(5):794-804.
[18] ZHAO H,WANG X,JIA Y,et al.The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance[J].Nature Communications,2018,9(1):2039.
[19] 章孟臣. 一个水稻稻瘟病抗性基因BRG8的克隆与功能分析[D].北京:中国农业科学院,2019.
[20] 马世伟. 水稻抗稻瘟病新基因的功能分析和Gα参与抗稻瘟病机理研究[D].福州:福建农林大学,2019.
[21] LIN F,CHEN S,QUE Z Q,et al.The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1[J].Genetics,2007,177(3):1871.
[22] KEIKO H,HITOSHI Y.Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter[J].Plant Journal,2010,57(3):413-425.
[23] TAKAHASHI A,HAYASHI N,MIYAO A,et al.Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging[J].BMC Plant Biology,2010,10(1):175.
[24] FUKUOKA S,YAMAMOTO S I,MIZOBUCHI R,et al.Multiple functional polymorphisms in a single disease resistance gene in rice enhance durable resistance to blast[J].Scientific Reports,2014,4(13):4550.
[25] MA J,LEI C,XU X,et al.Pi64,Encoding a Novel CC-NBS-LRR protein,confers resistance to leaf and neck blast in rice[J].Molecular Plant-Microbe Interactions,2015,28(5):558-568.
[26] WANG Z X,YANO M,YAMANOUCHI U,et al.The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes[J].Plant Journal,2010,19(1):55-64.
[27] XU X,HAYASHI N,WANG C T,et al.Rice blast resistance gene Pikahei-1(t),a member of a resistance gene cluster on chromosome 4,encodes a nucleotide-binding site and leucine-rich repeat protein[J].Molecular Breeding,2014,34(2):691-700.
[28] SHAOHONG Q,GUIFU L,BO Z,et al.The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J].Genetics,2006,172(3):1901-1914.
[29] ZHOU B,QU S G,DOLAN M,et al.The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J].Molecular Plant-Microbe Interactions,2006,19(11):1216-1228.
[30] CHEN X,SHANG J,CHEN D,et al.A B-lectin receptor kinase gene conferring rice blast resistance[J].Plant Journal,2010,46(5):794-804.
[31] SHANG J,TAO Y,CHEN X,et al.Identification of a new rice blast resistance gene,Pid3,by genomewide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes[J].Genetics,2009,182(4):1303-1311.
[32] JIE C,SHI Y,LIU W,et al.A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae [J].Journal of Genetics & Genomics,2011,38(5):209-216.
[33] LV Q M,XU X,SHANG J J,et al.Functional analysis of Pid3-A4,an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice[J].Phytopathology,2013,103(6):594-599.
[34] ZHU X,CHEN S,YANG J,et al.The identification of Pi50(t),a new member of the rice blast resistance Pi2/Pi9 multigene family[J].Theoretical & Applied Genetics,2012,124(7):1295.
[35] LIU X Q,LIN F,WANG L,et al.The in silico map-based cloning of Pi36,a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus[J].Genetics,2007,176(4):2541-2549.
[36] SANG-KYU L,MIN-YOUNG S,YOUNG-SU S,et al.Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes[J].Genetics,2009,181(4):1627.
[37] HIROKI T,AIKO U,HIROKI Y,et al.MutMap-Gap:whole-genome resequencing of mutant F2 progeny bulk combined with de novo assembly of gap regions identifies the rice blast resistance gene Pii[J].New Phytologist,2013,200(1):276-283.
[38] LIU Y,LIU B,ZHU X Y,et al.Fine-mapping and molecular marker development for Pi56(t),a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice[J].Theoretical & Applied Genetics,2013,126(4):985-998.
[39] SHARMA T R,RAI A K,GUPTA S K,et al.Broad-spectrum bast resistance gene Pi-kh cloned from rice Line Tetep designated as Pi54[J].Journal of Plant Biochemistry & Biotechnology,2010,19(1):87-89.
[40] IKUO A,NAGAO H,HIROKO Y,et al.Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance[J].Genetics,2008,180(4):2267-2276.
[41] CONCEPCION J C,FRANCESCHETTI M,MAQBOOL A,et al.Polymorphic residues in rice NLRs expand binding and response to effectors of the blast pathogen[J].Nature Plants,2018,4(8):576-585.
[42] NAGAO H,HARUHIKO I,TAKAHIRO K,et al.Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication[J].Plant Journal,2010,64(3):498-510.
[43] ZHAI C,LIN F,DONG Z Q,et al.The isolation and characterization of Pik,a rice blast resistance gene which emerged after rice domestication[J].New Phytologist,2011,189(1):321-334.
[44] YUAN B,ZHAI C,WANG W J,et al.The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes[J].Theoretical & Applied Genetics,2011,122(5):1017-1028.
[45] YUDAI O,HIROYUKI K,AKIRA A,et al.A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes[J].Plant Journal,2011,66(3):467-479.
[46] HUA L,WU J,CHEN C,et al.The isolation of Pi1,an allele at the Pik locus which confers broad spectrum resistance to rice blast Tag[J].Theoretical & Applied Genetics,2012,125(5):1047-1055.
[47] DAS A,SOUBAM D,SINGH P K,et al.A novel blast resistance gene,Pi54rh cloned from wild species of rice,Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae[J].Functional & Integrative Genomics,2012,12(2):215-228.
[48] CESARI S,THILLIEZ G,RIBOT C,et al.The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding[J].Plant Cell,2013,25(4):1463-1481.
[49] DEVANNA N B,VIJAYAN J,SHARMA T R.The blast resistance gene Pi54 of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains[J].PLoS One,2014,9(8):e104840.
[50] CHUN Z,YU Z,NAN Y,et al.Function and interaction of the coupled genes responsible for Pik-h encoded rice blast resistance[J].PLoS One,2014,9(6):e98067.
[51] ZHANG L,NAKAGOMI Y,ENDO T,et al.Divergent evolution of rice blast resistance Pi54 locus in the genus Oryza[J].Rice,2018,11(1):63.
[52] JING C,PEI P,TIAN J,et al.Pike,a rice blast resistance allele consisting of two adjacent NBS-LRR genes,was identified as a novel allele at the Pik locus[J].Molecular Breeding,2015,35(5):117.
[53] BRYAN G T,WU K S,FARRALL L,et al.A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J].Plant Cell,2000,12(11):2033-2045.
[54] WIJERATHNA Y,PERERA A N K,HAMAMA I B,et al.Application of PCR and MAS:potential use for assessment of genetic diversity of rice germplasm in breeding programmes in developing countries[J].Pertanika Journal of Tropical Agricultural Science,2015,38:161-174.
[55] KUROKAWA Y,NODA T,YAMAGATA Y,et al.Construction of a versatile SNP array for pyramiding useful genes of rice[J].Plant Science,2016,242:131-139.
[56] DAS G,PATRA J K,BAEK K H.Insight into MAS:A molecular tool for development of stress resistant and quality of rice through gene stacking[J].Front Plant Sciense,2017,8:985.
[57] ELLUR R K,KHANNA A,YADAV A,et al.Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding[J].Plant Science,2016,242:330-341.
[58] MIAH G,RAFII M Y,ISMAIL M R,et al.Marker-assisted introgression of broad-spectrum blast resistance genes into the cultivated MR219 rice variety[J].Journal of the Science of Food & Agriculture,2017,97(9):2810-2818.
[59] DAS G,RAO G J N,VARIER M,et al.Improved Tapaswini having four BB resistance genes pyramided with six genes/QTLs,resistance/tolerance to biotic and abiotic stresses in rice[J].Science Reports,2018,8(1):2413.
[60] TIAN D,GUO X,ZHANG Z,et al.Improving blast resistance of the rice restorer line,Hui 316,by introducing Pi9 or Pi2 with marker-assisted selection[J].Biotechnology & Biotechnological Equipment,2019,33(1):1195-1203.
[61] HUANG M,BALIMPONYA E G,MGONJA E M,et al.Use of genomic selection in breeding rice(Oryza sativa L.)for resistance to rice blast(Magnaporthe oryzae)[J].Molecular Breeding,2019,39(8):114.
[62] 裘烨. 利用分子标记辅助选择改良恢复系稻瘟病抗性的研究[D].长沙:湖南农业大学,2018.
[63] KUMARI M,RAI A K,DEVANNA B N,et al.Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae[J].Plant Cell Reports,2017,36(11):1747-1755.
[64] KUMARI M,DEVANNA B N,SINGH P K,et al.Stacking of blast resistance orthologue genes in susceptible indica rice line improves resistance against Magnaporthe oryzae [J].Biotechnology,2018,8(1):37.
[65] ZHOU H,HE M,LI J,et al.Development of commercial thermo-sensitive genic male serile rice accelerates hybrid rice breeding using the CRISPR/Cas9-mediated TMS5 editing system[J].Science Reports,2016,6:37395.
[66] SHAO G,XIE L,JIAO G,et al.CRISPR/CAS9-mediated editing of the fragrant gene Badh2 in rice[J].Chinese Journal of Rice Science,2017,31(2):216-222.
[67] SUN Y,JIAO G,LIU Z,et al.Generation of high-amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes[J].Frontiers in Plant Science,2017,223(8):298.
[68] TANG L,MAO B G,LI Y K,et al.Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulatingindicarice without compromising yield[J].Scientific Reports,2017,7(1):14438.
[69] ZHANG J,ZHANG H,BOTELLA J R,et al.Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties[J].Journal of Integrative Plant Biology,2018,60(5):369-375.
[70] LI S,SHEN L,HU P,et al.Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing[J].Journal of Integrative Plant Biology,2019,61(12):1201-1205.
[71] XIE Z,YAN B,SHOU J,et al.A nucleotide-binding site-leucine-rich repeat receptor pair confers broad-spectrum disease resistance through physical association in rice[J].Philosophical Transactions of The Royal Society of London,2019,374(1767):20180308.
[72] KHAN M H,DAR Z A,DAR S A.Breeding strategies for improving rice yield[J].Agricultural Sciences,2015,6(5):467-478.

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