9种真菌中分支点结合蛋白的生物信息学分析

doi:10.12190/j.issn.2096-1197.2020.03.06

Abstract

Abstract: 【Objective】To further analysis of the biological functions of branchpoint-bridging protein （BBP） in fungi.【Methods】A variety of bioinformatics software and websites are used to predict and analyze the BBP in nine fungi （Pyricularia oryzae、Saccharomyces cerevisiae、Gaeumannomyces tritici、Neurospora crassa、Colletotrichum orbiculare、Beauveria bassiana、Fusarium oxysporum、Fusarium fujikuroi and Rhizoctonia solani）,including the basic information of the protein and gene,the physical and chemical properties,the subcellular localization and signal peptide and protein conformation and transmembrane helical structure,phosphorylation site on a SUMO loci,and build the system of the evolutionary tree.【Results】The molecular weight of BBP range from 50 812.65 Da to 68 306.99 Da.They were all unstable hydrophilic proteins located in the nucleus.There were no signal peptides and transmembrane helixes.Alpha helix was the main secondary structure and SF1-HH,KH_dom and Znf_CCHC domains exist in all BBPs of the nine fungi.However,ONH80283.1 of Saccharomyces cerevisiae and CCO28600.1 of Rhizoctonia solani showed different molecular evolution and spatial conformation to BBPs of other fungi.The phosphorylation types and percentages of BBPs were similar in the nine fungi,but CCO28600.1 had more SUMO sites.【Conclusion】Among the nine common fungi,the BBP structure of Saccharomyces cerevisiae and Rhizoctonia solani was relatively special,and the evolution of BBP was relatively conservative in Pyricularia oryzae,Gaeumannomyces tritici,Neurospora crassa,Colletotrichum orbiculare,Beauveria bassiana,Fusarium oxysporum and Fusarium fujikuroi.

Key words: Branchpoint-bridging protein, Fungi, Bioinformatics analysis, SUMO

CLC Number:

Q949.32

QIU Kaihua, LI Xiaoju, FANG Shumei, LIANG Xilong. Bioinformatic analysis of branchpoint-bridging protein in nine fungi[J].Journal of Northern Agriculture, 2020, 48(3): 39-46.

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References 24

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Bioinformatic analysis of branchpoint-bridging protein in nine fungi

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