籼稻资源稻瘟病抗性基因的分子检测与抗性分析

doi:10.12190/j.issn.2096-1197.2025.02.01

Abstract

Abstract: 【Objective】To investigate the distribution of five rice blast resistance genes（Pi2,Pib,Pita,Pigm,Pi9） in 86 indica rice accessions and evaluate the resistance effects of different genotypic combinations,providing a theoretical basis for breeding rice blast-resistant varieties and effective pyramiding of resistance genes.【Methods】Using five pairs of functional markers with clear amplification bands,blast resistance genes in 86 indica rice accessions from the Huang-Huai rice region were detected,and combined with artificial inoculation assays,the distribution of five resistance genes and the genotypic combinations conferring moderate to high resistance in this region were identified.【Results】The distribution frequencies of the five resistance genes Pi2,Pib,Pita,Pigm and Pi9 in the 86 indica rice accessions were 98.80%,46.50%,55.80%,48.80%,and 1.20%,respectively. Ten genotypic combinations were identified among the 86 tested materials,no resistance genes,Pi2,Pi2+Pita,Pi2+Pib,Pi2+Pigm,Pi2+Pib+Pita,Pi2+Pi9+Pigm,Pi2+Pib+Pigm,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm,respectively. Inoculation assays showed that 33 accessions exhibited moderate or higher resistance,accounting for 38.37%;among the materials with three genotypic combinations Pi2+Pib+Pita,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm,the proportions of accessions with moderate or higher resistance were 50%,80% and 100%,respectively.【Conclusion】The Pi2 gene exhibits the highest distribution frequency（98.80%） among the 86 indica rice accessions;the three genotypic combinations conferring superior resistance are Pi2+Pib+Pita,Pi2+Pita+Pigm and Pi2+Pib+Pita+Pigm.

Key words: Indica rice, Rice blast, Resistance genes, Resistance analysis, Genotype

CLC Number:

S435.111.41

CHEN Chun, LI Fan, GUO Xinya, WANG Feifei, WANG Lei, CHEN Weijun. Molecular detection and resistance analysis of rice blast resistance genes in indica rice accessions[J].Journal of Northern Agriculture, 2025, 53(2): 1-9.

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Molecular detection and resistance analysis of rice blast resistance genes in indica rice accessions

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