154份荞麦荧光特性评价及高光效种质筛选

doi:10.12190/j.issn.2096-1197.2025.05.04

Abstract

Abstract: 【Objective】 To explore the effect of key fluorescence parameters on the characterizing value of high light-use efficiency in buckwheat and to screen high light-use efficiency buckwheat germplasm through fluorescence characteristic evaluation,providing a scientific basis and material support for high light-use efficiency breeding.【Methods】 A randomized block field experiment was conducted,using 154 buckwheat varieties as experimental materials. Twenty-five chlorophyll fluorescence parameters were measured. Correlation analysis,principal component analysis （PCA）,and systematic clustering-shortest distance analysis were employed to comprehensively evaluate the fluorescence characteristics of buckwheat and identify superior germplasm resources with high light-use efficiency.【Results】 Highly significant differences were observed in chlorophyll fluorescence parameters among different buckwheat varieties（P<0.01）. Specifically,parameters PI abs、PI total,ETo/CSm,DIo/RC,REo/CSm,Fv/Fo,TRo/CSm,Fv and Fo/Fm showed larger coefficients of variation（CV） values,all exceeding 25.00%. Parameters DF total,DF abs,TRo/RC,DIo/CSm and Fo showed smaller CV values,ranging from 0.71% to 7.97%. Broad-sense heritability of all other parameters,except TRo/RC,REo/RC,REo/CSo and PI total,was greater than 60.00%. Principal component analysis revealed that the cumulative contribution rate of the top four principal components reached 96.758%,and a comprehensive evaluation function model was established as A=0.612A₁+0.190A₂+0.130A₃+0.068A₄,with A values for the 154 buckwheat varieties ranging from -2.68 to 1.20. Clustering analysis of the A values classified the tested varieties into five categories：2 buckwheat varieties（NQ148 and NQ150） were classified as the high light-use efficiency germplasm type,105 varieties were classified as the relatively high light-use efficiency germplasm type,44 varieties were classified as the moderate light-use efficiency germplasm type,2 varieties were classified as the relatively low light-use efficiency germplasm type,and 1 variety was classified as the low light-use efficiency germplasm type.【Conclusion】 Two buckwheat varieties（NQ148 and NQ150） with high light-use efficiency were successfully screened,providing materials for the breeding of high light-use efficiency germplasm resources in buckwheat.

Key words: Buckwheat, Chlorophyll, Fluorescence characteristics, High light-use efficiency germplasm, Comprehensive evaluation

CLC Number:

S517

HU Haochi, WU Yening, XIE Rui, LIU Jinchuan, WEN Rui, WANG Zhichao, WANG Chunyong, YU Chuanzong, JIN Xiaolei. Evaluation of fluorescence characteristics and screening of high light-use efficiency germplasm in 154 buckwheat varieties[J].Journal of Northern Agriculture, 2025, 53(5): 36-48.

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Evaluation of fluorescence characteristics and screening of high light-use efficiency germplasm in 154 buckwheat varieties

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