Abstract: In order to clarify the difference of drought resistance of gramineous forage seedling varieties under repeated drought treatment, 10 gramineous forage varieties were selected, and the emergence rate, root-shoot ratio, electrical conductivity ,chlorophyll content and peroxidase activity of each forage variety were analyzed and compared. The results showed that the emergence rate of Elytrigia trichophora （Ling） Nevsky. was the largest, the emergence rate of Thinopyrum pontivum（Alker） was the lowest, the root-shoot ratio of the （Agropyron desertorum （Fisch.） Schult.） was the largest and the root-shoot ratio of Thinopyrum pontivum （Alker） was the lowest. The highest electric conductivity of Thinopyrum pontivum （Alker） and the lowest of the electric conductivity of Thinopyrum pontivum （Jose）, the largest chlorophyll content of the grass variety is the Agropyron desertorum （Fisch.） Schult., the smallest forage species is Thinopyrum pontivum （Alker）, The activity of peroxidase of the Agropyron desertorum （Fisch.） Schult. was the largest and the lowest in Elytrigia repens（L.） Dasv. The results of comprehensive evaluation showed that the seedlings of Agropyron desertorum （Fisch.） Schult., Leymus racemasus, Elytrigia trichophora （Ling）Nevsky., Thinopyrum pontivum （Jose） had strong drought resistance, while the seedlings of Thinopyrum pontivum （Tyrell）, Thinopyrum pontivum（Alker）, Elytrigia repens（L.）Desv were less resistant to drought. The drought resistance of the other three forage varieties was between 7 forage varieties.