高丹草组织培养再生体系的建立

doi:10.12190/j.issn.2096-1197.2020.05.21

Abstract

Abstract: 【Objective】To establish a tissue culture and regeneration system of Sorghum-Sudangrass for the research of Sorghum-Sudangrass transgenic breeding.【Methods】The experiment used MS as the basic medium,and the mature seeds of Sorghum-Sudangrass were used as explants to study the effect of different exogenous hormone ratios（2,4-D,NAA,6-BA,IBA）on callus,induction and differentiation of adventitious buds and influence of seedling rooting.【Results】The suitable callus induction medium was MS+2,4-D（1.5 mg/L）,and the induction rate reached 65.63%;adventitious bud inductions MS medium with NAA（0.5 mg/L）and 6-BA（1.5 mg/L）was optimal,with a adventitious bud induction rate of 80.21%;the optimal rooting medium was MS medium containing 1.0 mg/L IBA,with a rooting rate of 74.30%;for transplanting tissue culture seedlings,the nutrient soil of flower soil∶perlite∶vermiculite = 3∶1∶1 was selected as the transplanting substrated.【Conclusion】 The regeneration system of mature seeds of Sorghum-Sudangrass was induction medium MS+2,4-D（1.5 mg/L）,and the bud differentiation medium was MS+NAA（0.5 mg/L）+6-BA（1.5 mg/L）,and rooting medium was MS+IBA（1.0 mg/L）.

Key words: Sorghum-Sudangrass, Mature seeds, Tissue culture, Callus

CLC Number:

S544.9

SHI Yue, FANG Yongyu, YU Zhuo, YANG Dongsheng, CHAI Wenjuan, LIU Shuancheng. Establishment of tissue culture and regeneration system for Sorghum-Sudangrass[J].Journal of Northern Agriculture, 2020, 48(5): 119-124.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2020.05.21

https://journal30.magtechjournal.com/bfnyxb/EN/Y2020/V48/I5/119

References

[1] 于卓,马艳红,李小雷,等.蒙农3号高丹草选育[J].中国草地学报,2008,30(6):1-9.
[2] 詹秋文,钱章强.高粱与苏丹草杂种优势利用的研究[J].作物学报,2004,30(1):73-77.
[3] 于卓,山田敏彦.高丹草品种主要农艺性状的比较研究[J].中国草地学报,2006,28(6):1-6.
[4] 李源,谢楠,赵海明,等.高丹草营养生长与饲用品质变化规律分析[J].草地学报,2011,19(5):813-820.
[5] 霍秀文. 园艺植物组织培养实验指导[M].呼和浩特:内蒙古农业大学,2005.
[6] CHEN Q,JAHIER J,CAUDERON Y.Production of embryo-callus-regenerated hybrids between Triticum aestivum and Agropyron cristatum possessing one B chromosome[J].Agronomie,1992,12(7):551-555.
[7] 刘明,赵琦.高粱组织培养研究进展及关键技术[J].首都师范大学学报,2005,26(4):60-63.
[8] CAI T,DALY B,BUTLER L.Callus induction and plant regeneration from shoot portions of mature embryos of high tannin sorghums[J].Palnt Cell Tissue and Organ Culture,1987,9(3):245-252.
[9] 李杰勤,王丽华,詹秋文,等.两个苏丹草品系成熟种子的组织培养和植株再生研究[J].广西植物,2016,36(8):930-936.
[10] 于卓,秦永梅,赵晓杰,等.优质饲用作物新品种—蒙农青饲1号高丹草选育[J].中国草地,2004,26(2):1-9.
[11] 于卓,刘永伟,赵晓杰,等.高粱11A与3种苏丹草杂种F₁的农艺特性及细胞遗传学研究[J].草业学报,2006,15(1):90-96.
[12] 房永雨,于肖夏,于卓,等.低氰含量高丹草新品系主要农艺特性及染色体构型分析[J].草业学报,2012,23(2):162-170.
[13] 石悦,于肖夏,于卓,等.高丹草AFLP分子遗传连锁图谱的构建[J].西北农林科技大学学报,2017,45(10):7-14.
[14] 谭化,王中伟,周紫阳,等.高粱幼穗离体培养再生体系的建立[J].吉林农业科学,2008,33(1):23-25.
[15] 艾克拜尔·毛拉,彭媛媛,王海鸥,等.鞑靼忍冬和疏花蔷薇组培快繁体系的建立研究[J].种子,2020,39(2):26-31.
[16] 刘志华,马艳红,曹枫.罗布麻种子组培再生体系的研究[J].种子,2010,29(4):5-8.
[17] 吕剑,喻景权.植物生长素的作用机制[J].植物生理学通讯,2004,40(5):624-628.
[18] 程泉,雷中华,黄启秀,等.油用向日葵亲本‘早矮大头’的组织培养与植株再生体系的建立[J].植物生理学报,2020,56(2):180-188.
[19] 罗芃睿,张帆,崔杰,等.迷你岩桐组织培养及植株再生体系建立[J].草业科学,2020,37(3):469-476.

Establishment of tissue culture and regeneration system for Sorghum-Sudangrass

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	PENG Boyang, BAI Ruiqin, LYU Yanfang, FAN Xudong, HU Yuejun, LYU Meiye. Establishment of in vitro rapid propagation system of two edible lily cultivars [J]. Journal of Northern Agriculture, 2022, 50(6): 88-97.
[2]	HUANG Weili, ZHANG Wenjun, ZHANG Haidong, YANG Yuewen, ZHANG Fenghe, ZHANG Wen. Study on rapid propagation system of Xanthoceras sorbifolia tissue culture [J]. Journal of Northern Agriculture, 2022, 50(4): 102-108.
[3]	DENG Yu, WANG Meng, LI Fahu, LI Longmei, HOU Jia. Comparative study on surface sterilization methods for Lilium pumilum seed [J]. Journal of Northern Agriculture, 2022, 50(1): 87-92.
[4]	TAN Yiluo, CHEN Feng, ZHANG Guangxu, WANG Kangjun, GUO Mingming, LI Xiaofeng, ZHANG Menghan, FAN Jiwei. Callus induction and regeneration system optimization of color wheat mature embryos [J]. Journal of Northern Agriculture, 2021, 49(5): 25-31.
[5]	YUAN Rui, TANG Wei, SUN Shujun, ZHAO Lukuan, HU Yang, MA Jukui, WANG Jie, CAO Qinghe, ZHOU Zhilin. Study on rapid propagation and transplanting of tissue culture seedlings of sweet potato with SPVD removed [J]. Journal of Northern Agriculture, 2021, 49(5): 112-117.
[6]	HU Binjie, BAI Ruiqin, YIN Jingjing, WANG Hongbin, HU Yuejun. Construction of tissue culture and rapid propagation system of Yishui edible Lilium brownii var. viridulum [J]. Journal of Northern Agriculture, 2021, 49(3): 90-95.
[7]	LIU Wei, LIU Bo, CAI Weijia, WANG Hao, CHEN Fen, TAN Jun. Research progress of cultivars of soft-seed pomegranate in domestic [J]. Journal of Northern Agriculture, 2020, 48(4): 75-82.
[8]	TAN Jun, LUO Guijie, MA Bailin, LIU Bo, YIN Qing. Establishment of the in vitro culture and rapid propagation system of fruit mulberry [J]. Journal of Northern Agriculture, 2019, 47(6): 130-134.
[9]	XIONG Qin, FU Sujing, LU Wangui. Callus induction and plant regeneration of Zephyranthes candida [J]. Journal of Northern Agriculture, 2019, 47(5): 82-85.
[10]	ZHOU Lingling;ZHANG Lijie;TIAN Fufa;JIANG Ruoyong(Suqian Institute of Agricultural Sciences,Jiangsu Academy of Agricultural Sciences,Suqian 223800,China). Study on the purification and rejuvenation technology in daylily Da Wu Zui with local characteristics [J]. Journal of Northern Agriculture, 2019, 47(2): 111-114.
[11]	. Establishment of rapid propagation system of Dracocephalum rupestre Hance In Vitro Culture [J]. Journal of Northern Agriculture, 2017, 45(6): 102-102.
[12]	. Rapid propagation technology system of Ulmus pumila tissue culture [J]. Journal of Northern Agriculture, 2017, 45(6): 107-107.
[13]	. Study on technical optimization of preventing browning for strawberry in stem-tip culture [J]. Journal of Northern Agriculture, 2016, 44(3): 33-33.
[14]	. Induction of callus and plant regeneration from shoot tip in Agropyron mongolicum Keng [J]. Journal of Northern Agriculture, 2016, 44(2): 18-18.
[15]	. The optimization of different factors on tissue culture and rapid propagation of Dianthus Linn [J]. Journal of Northern Agriculture, 2016, 44(2): 23-23.