向日葵种子甲基磺酸乙酯诱变体系优化与分析

doi:10.12190/j.issn.2096-1197.2021.06.02

Abstract

Abstract: 【Objective】To determine the optimal ethyl methane sulfonate（EMS）mutagensis concentration and treatment time frame in sunflower seeds.【Methods】Using oil-seed sunflower JK103,S18 and edible sunflower Bakui 138 and Keyang No.7 as test materials,the germination potential,germination rate,and relative germination rate of sunflower seeds were analyzed under EMS treatment with gradient concentration（0,0.1%,0.5%,1.0%,1.5%,2.0%）and three treatment time span（6,12,24 h）to determine the optimal conditions.【Results】The germination potential and germination rate of sunflower seeds were affected by the EMS solution concentration and mutagenic time. Sunflower seeds,especially oil-seed was quite tolerant to EMS. In the later stages of mutagensis,edible seeds became more sensitive to EMS as the treatment duration increases. The optimal mutagenic conditions of sunflower EMS treatment varied depending on the variety.【Conclusion】According to the principle of semi-lethal condition,the optimal concentration and treatment time are,for oil-seed,48 h after germination treated with 1.0%-1.5% EMS solution for 12-24 h,for edible-seed,48-72 h after germination treated with 1.0%-1.5% EMS solution for 6-12 h.

Key words: Sunflower, Seed, EMS mutagensis, Germination potential, Germination rate

CLC Number:

GUO Shuchun, MIAO Hongmei, ZHANG Yanfang, YU Haifeng, NIE Hui, SHAO Ying, QIAO Huilei, MU Yingnan, LIANG Chen, LI Suping. Optimization and analysis of ethyl methane sulfonate mutagensis system in sunflower seeds[J].Journal of Northern Agriculture, 2021, 49(6): 15-21.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2021.06.02

https://journal30.magtechjournal.com/bfnyxb/EN/Y2021/V49/I6/15

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Optimization and analysis of ethyl methane sulfonate mutagensis system in sunflower seeds

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