水杨酸诱导水稻抗稻瘟病的机制研究

doi:10.12190/j.issn.2096-1197.2022.04.07

Abstract

Abstract:

【Objective】To clarify the molecular mechanism of salicylic acid induced rice blast resistance.【Methods】Using Hwayoung rice seedlings as test material,the in vitro inhibitory effect of salicylic acid on Magnaporthe oryzae was evaluated. Rice seedlings were induced by salicylic acid(sterile water as control)and then inoculated with Magnaporthe oryzae JC2. The changes in histone acetylation and gene expression related to rice disease course were studied.【Results】Salicylic acid had no significant inhibitory effect on the in vitro growth rate,spore quantity,and spore germination rate of Magnaporthe oryzae. The histone modification levels of H3K9ac and H4K5ac showed an increased trend when the rice seedlings were induced by different concentrations of salicylic acid,and the resistance to rice blast was also improved. Compared with the control plants,in 0.05 mmol/L salicylic acid induced and inoculated 2 d plants(sampled 24 h after inoculation),the expression levels of OsPR1b、OsPAL、OsNH1 and WRKY13 increased extreme significantly(P<0.01).【Conclusion】After rice plants were induced by salicylic acid,the histone acetylation modification level was elevated and the resistance to rice blast was improved.

Key words: Rice, Salicylic acid, Rice blast, Induced resistance, Histone acetylation

CLC Number:

S435.111.41

FU Jia, LIU Tingting, ZHANG Hui, JIANG Shanqiang, ZHOU Li, MA Dan, TIAN Xuejun, ZHU Deyan, XU Yan. Study on the mechanism of salicylic acid inducing rice blast resistance[J].Journal of Northern Agriculture, 2022, 50(4): 47-54.

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URL: https://journal30.magtechjournal.com/bfnyxb/EN/10.12190/j.issn.2096-1197.2022.04.07

https://journal30.magtechjournal.com/bfnyxb/EN/Y2022/V50/I4/47

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Study on the mechanism of salicylic acid inducing rice blast resistance

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