畜牧与饲料科学 ›› 2010, Vol. 31 ›› Issue (3): 13-13.doi: 10.12160/j.issn.1672-5190.2010.03.006

• 基础科学 • 上一篇    下一篇

奶牛神经肽Y基因的克隆及测序

吴笳笛[1] 费东亮[1] 张才[2]   

  1. [1]辽宁医学院畜牧兽医学院,辽宁锦州121001 [2]河南科技大学动物科学技术学院,河南洛阳471003
  • 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 吴笳笛
  • 作者简介:吴笳笛(1972-),女,副教授,硕士,主要研究方向为动物生物化学。
  • 基金资助:
    辽宁省教育厅资助项目(2009B121)

Cloning and Sequencing of Neuropeptide Y Gene of Dairy Cows

WU Jia-di,FEI Dong-liang,ZHANG Cai (1.College of Animal Husbandry and Veterinary Medicine,Liaoning Medical University,Jinzhou 121001,China;2.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China)   

  • Online:2010-03-20 Published:2010-03-20

摘要: 根据GenBank中已公布的奶牛神经肽Y(neuropeptide Y,NPY)基因序列设计引物,应用聚合酶链式反应(polymerase chain reaction,PCR),以犊牛丘脑组织cDNA为模板,扩增出奶牛NPY的全基因序列,并对其进行了克隆、测序及序列分析。结果表明,试验所设计的引物可以成功用于扩增奶牛NPY的全基因序列,所扩增的序列与GenBank中已有序列的同源性达100%。

Abstract: According to the sequence of neuropeptide Y (NPY) gene published in GenBank,a pair of primers was designed.With cDNA extracted from calf hypothalamus as a template,NPY gene was amplified by polymerase chain reaction (PCR).The PCR products were cloned,sequenced and analyzed.The results showed that the designed primers could be used to amplify NPY gene of cow,and the amplified NPY gene had 100% nucleic acid homology to that published in GenBank.

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