关键词: 醇溶蛋白基因;产朊假丝酵母;载体构建

Abstract: The present study was designed to construct a genetically engineered strain of Candida utilis that expresses methionine-rich zein protein. By using genetic engineering method, a methionine-rich δ-10kD-zein gene of maize endosperm, the promoter （GA P-p） gene and the terminator （GAP-t） gene from yeast were spliced into an expression cassette; subsequently, a homologous recombinant expression vector was constructed with a 18S rDNA fragment （serving as an integrated mediated region）, a cycloheximide （CYH） resistance gene （serving as a selection marker） and the pBR322 plasmid; the zein gene was integrated into the chromosome of Candida utilis by using homologous recombination. The determination results of methionine content by HPLC demonstrated that the expression of methionine in the recombinant strain was increased by 17.14% than the wild one. The constructed genetically engineered strain of Candida utilis provided technological foundation for further study on increasing its production of methionine.