畜牧与饲料科学 ›› 2011, Vol. 32 ›› Issue (11): 25-25.doi: 10.12160/j.issn.1672-5190.2011.11.012

• 动物营养与饲料科学 • 上一篇    下一篇

DGGE技术的原理及其在动物胃肠道微生态系统研究中的应用

鲍新宇[1] 杨剑[2] 高新艳[2] 周勤飞[1] 王永才[1]   

  1. [1]西南大学荣昌校区动物科技学院,重庆402460 [2]西南大学荣昌校区动物医学系,重庆402460
  • 出版日期:2011-11-20 发布日期:2011-11-20
  • 通讯作者: 鲍新宇
  • 作者简介:鲍新宇(1985-),女,硕士,主要研究方向为单胃动物营养。 通讯作者:王永才(1961-),男,教授,硕士生导师,主要研究方向为畜牧技术、毛皮加工、包装设计。

Principle of DGGE and Its Application in Animal Gastrointestinal Microbiota System

BAO Xin-yu, YANG Jian, GAO Xin-yan, ZHOU Qin-fei, WANG Yong-cai (1.College of Animal Science of Technology, Rongehang School Area of Southwest University, Chongqing 402460, China;2. Department of Veterinary Medicine, Rongchang School Area of Southwest University, Chongqing 402460, China)   

  • Online:2011-11-20 Published:2011-11-20

摘要: 利用传统的培养方法很难将动物胃肠道的所有菌群进行培养,因此也很难鉴别各个时期动物胃肠道微生物之间的差异。变性梯度凝胶电泳(DGGE)技术可以避免传统方法的弊端,进而可以进一步确定动物胃肠道微生态系统的差别以及茵群的多样性。主要综述了DGGE技术的原理、优缺点以及在动物胃肠道微生态系统研究中的应用和前景。

Abstract: It is difficult to cultivate all flora in animal gastrointestinal tract by using traditional methods, so it is difficult to identify the difference of animal gastrointestinal microbes in each stage. Denaturing gradient gel electrophoresis (DGGE) can avoid the disadvantages of traditional methods and can confirm the difference in animal gastrointestinal microbiota system and the diversity of flora. The basic principle, advantages and disadvantages of DGGE and its application in animal gastrointestinal microbiota system were mainly summarized in this paper.

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