Abstract: The fermentation technology of chromium-enriched yeast produced by using Candida utilis was studied.The chromium-enriched yeast was prepared by adding Cr3＋ in fermentation culture medium.The biomass and chromium content of chromium-enriched yeast were determined,and the fermentation culture conditons were optimized.The optimal culture conditions were as follows：the cultural temperature was 30 ℃;the the addition amount of Cr3＋ in culture medium was 100 μg/mL;the inoculation amount was 4%-6%（V/V）;the initial pH value was 5.0;and the cultural time was 20 h.Under the optimal conditions,the biomass of chromium-enriched yeast and organic chromium content were 1.83 g/100mL and 2 210 μg/g,respectively.The results lay the technology foundation for the development of chromium-enriched yeast.