畜牧与饲料科学 ›› 2019, Vol. 40 ›› Issue (3): 12-15.doi: 10.12160/j.issn.1672-5190.2019.03.003

• 著者文摘 • 上一篇    下一篇

犬药物性急性肾损伤模型的建立及评价

聂静[1];仇爽[1];朱婷婷[1];韦人月[1];阮红日[1];魏雪[1];郑家三[1]   

  1. [1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319
  • 收稿日期:2019-01-12 出版日期:2019-03-30 发布日期:2019-08-19
  • 通讯作者: 聂静-黑龙江八一农垦大学动物科技学院,黑龙江大庆163319
  • 作者简介:聂静(1992—),女,硕士研究生,主要研究方向为小动物疾病学;通讯作者:郑家三(1978-),男,副教授,博士,主要研究方向为奶牛肢蹄病和小动物外科学。
  • 基金资助:
    国家重点研发计划项目(2016YFD0501008)。

Establishment and Evaluation of a Drug-induced Acute Renal Injury Model in Dog

NIE Jing[1];QIU Shuang[1];ZHU Ting-ting[1];WEI Ren-yue[1];RUAN Hong-ri[1];WEI Xue[1];ZHENG Jia-san[1]   

  1. [1]College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China
  • Received:2019-01-12 Online:2019-03-30 Published:2019-08-19

摘要: [目的]探讨建立犬药物性急性肾损伤模型。[方法]16只健康成年比格犬每8h皮下注射20mg/kg庆大霉素,注射前血肌酐浓度(SCr)作为基线,注射后每6h采集血液检测SCr浓度,于SCr无明显升高、SCr升高临近正常值上限及SCr升高到基线1.5倍时观察肾组织病理形态学改变,确定犬药物性急性肾损伤模型。[结果]SCr在注射庆大霉素后78h显著升高(P<0.05),实验犬SCr均在120~132h达到基线的1.5倍;肾组织在SCr升高到基线1.5倍时肾皮质大面积的肾小管变性、坏死,管腔有较多的细胞碎片和颗粒管型,基底膜裸露明显,髓质损伤较轻;与健康对照组相比,注射后肾损伤评分差异有统计学意义(P<0.05),且肾损伤随用药时间延长评分逐渐增加。[结论]庆大霉素每8h皮下注射20mg/kg直至SCr浓度升高到基线1.5倍的方法可成功建立犬药物性急性肾损伤模型。

关键词: 犬, 药物性急性肾损伤, 模型

Abstract: [Objective] To establish a drug-induced acute kidney injury model in dog.[Method] A total of 16 healthy adult beagle dogs were subcutaneously injected with 20 mg/kg gentamicin every 8 h.The serum creatinine concentration (SCr) before injection was used as the baseline,and the blood samples were recovered every 6 h after injection to determine the SCr.When there was no significant increase in SCr,SCr increased near the upper limit of normal value,and SCr increased 1.5 times to baseline,the pathological changes of renal tissue were observed,and the model of canine drug-induced acute kidney injury was determined.[Result] SCr was significantly increased at 78 h post gentamicin injection (P<0.05).The SCr of the tested dogs reached 1.5 times of the baseline during 120 h to 132 h post injection.When the SCr was 1.5 times higher than that of the baseline,the following pathological changes were observed: extensive degeneration and necrosis of renal tubules in renal cortex,increased cell debris and granules in lumen of renal tubules,the bare basement membrane,and the minor damage in renal medulla.Compared with the healthy controls,the difference in renal injury score after injection was statistically significant (P<0.05),and the renal injury gradually deteriorated with the prolonged medication time.[Conclusion] A model of canine drug-induced acute kidney injury can be successfully established by subcutaneous injection of 20 mg/kg of gentamicin every 8 h till the SCr was 1.5 times higher than that of the baseline.

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