益生菌牙膏缓解犬牙菌斑和牙结石的研究

doi:10.12160/j.issn.1672-5190.2022.05.019

畜牧与饲料科学 ›› 2022, Vol. 43 ›› Issue (5): 117-122.doi: 10.12160/j.issn.1672-5190.2022.05.019

益生菌牙膏缓解犬牙菌斑和牙结石的研究

郑智杰^1,²,彭曦冉^1,²,焦玉珠³,唐海丽³,陈欣雨³,张晓露³,王自力^1,²,吴海冲^1,²

1.浙江大学动物医学中心，浙江杭州 310058
2.浙江大学动物科学学院，浙江杭州 310058
3.上海弗艾柏生物科技有限公司，上海 200092

收稿日期:2022-06-24 出版日期:2022-09-30 发布日期:2022-09-21
通讯作者: 吴海冲（1989—），男，副研究员，博士，硕士生导师，主要研究方向为小动物疾病。
作者简介:郑智杰（1999—），男，硕士研究生，主要研究方向为小动物疾病。
基金资助:
宠物临床药物新技术评价试验项目(581420-I22001)

Efficacy Evaluation of a Probiotic Toothpaste in Relieving Canine Dental Plaque and Calculus

ZHENG Zhi-jie^1,²,PENG Xi-ran^1,²,JIAO Yu-zhu³,TANG Hai-li³,CHEN Xin-yu³,ZHANG Xiao-lu³,WANG Zi-li^1,²,WU Hai-chong^1,²

1. Animal Medicine Center，Zhejiang University，Hangzhou 310058，China
2. College of Animal Sciences，Zhejiang University，Hangzhou 310058，China
3. Shanghai Fiber Biotech Co.，Ltd.，Shanghai 200092，China

Received:2022-06-24 Online:2022-09-30 Published:2022-09-21

摘要/Abstract

摘要：

[目的]评价某公司生产的一款宠物用益生菌牙膏对犬牙菌斑和牙结石形成的缓解功效。[方法]选取口腔健康、体况良好的成年比格犬10只，配对设计随机分为阴性对照组（n=5）和益生菌牙膏组（n=5）；阴性对照组仅饲喂犬粮，益生菌牙膏组每次餐后给予约4 g/只益生菌牙膏，每天2次，连续使用28 d；试验第0天对所有试验犬牙齿进行洁牙抛光处理，试验第14天和第29天分别对比格犬牙菌斑进行评分，第29天对牙结石进行评分并检测口腔气体挥发性化合物（H₂S+CH₃SH）浓度；当牙菌斑和牙结石评分均值以及口腔气体挥发性化合物浓度均值减少幅度≥15%时，定义为益生菌牙膏效果显著。[结果]与阴性对照组相比，益生菌牙膏组在给予益生菌牙膏第14天时，牙菌斑评分均值减少幅度为-0.36%（P>0.05），第29天牙菌斑评分均值减少幅度为21.47%（P<0.05）；第29天益生菌牙膏组牙结石评分均值减少幅度为6.54%（P>0.05）；第29天益生菌牙膏组犬口腔气体挥发性化合物浓度均值减少幅度为-44.59% （P>0.05）。[结论]在试验期内，该款益生菌牙膏能够有效抑制犬牙菌斑的形成，但不具有减少牙结石形成和降低口腔气体挥发性化合物浓度的功效。

关键词: 益生菌牙膏, 犬, 牙菌斑, 牙结石

Abstract:

[Objective] This study was conducted to evaluate the efficacy of a probiotic toothpaste for pet use produced by a company in relieving the formation of dental plaque and calculus in dogs. [Method] Ten adult Beagle dogs with healthy oral cavity and good physical condition were randomly divided into a negative control group （n=5） and a probiotic toothpaste group （n=5）. The negative control group was fed with dog food alone, and the probiotic toothpaste group was given about 4 g probiotic toothpaste per dog twice a day for consecutive 28 days after each meal. The teeth of all the experimental dogs were cleaned and polished on day 0 of experiment. The dental plaque were scored on day 14 and day 29 of experiment, respectively. The dental calculus and the concentrations of volatile compounds （H₂S+CH₃SH） in oral gas were scored and assessed on day 29 of experiment, respectively. Probiotic toothpaste was defined as having a significant efficacy when the mean values of scores regarding dental plaque and calculus formation as well as those of concentrations of volatile compounds in oral gas were reduced by 15% or higher. [Result] Compared with the control group, the mean values of scores regarding dental plaque formation of the probiotic toothpaste group were decreased by -0.36% （P>0.05） and 21.47% （P<0.05） on day 14 and day 29 of experiment, respectively; the mean values of score regarding dental calculus formation and concentrations of volatile compounds in oral gas of the probiotic toothpaste group were dropped by 6.54% （P>0.05） and -44.59% （P>0.05） on day 29 of experiment, respectively. [Conclusion] Within the experimental period, the tested probiotic toothpaste effectively inhibited the formation of dental plaque in Beagle dogs, but had no efficacy on relieving the formation of dental calculus and reducing the concentrations of volatile compounds in oral gas.

Key words: probiotic toothpaste, canine, dental plaque, dental calculus

中图分类号:

S858.292

郑智杰, 彭曦冉, 焦玉珠, 唐海丽, 陈欣雨, 张晓露, 王自力, 吴海冲. 益生菌牙膏缓解犬牙菌斑和牙结石的研究[J]. 畜牧与饲料科学, 2022, 43(5): 117-122.

ZHENG Zhi-jie, PENG Xi-ran, JIAO Yu-zhu, TANG Hai-li, CHEN Xin-yu, ZHANG Xiao-lu, WANG Zi-li, WU Hai-chong. Efficacy Evaluation of a Probiotic Toothpaste in Relieving Canine Dental Plaque and Calculus[J]. Animal Husbandry and Feed Science, 2022, 43(5): 117-122.

图/表 12

表1

表2

图1

图2

图3

图4

表3

表4

图5

表5

表6

图6

参考文献 15

[1]	ÖZAVCI V, ERBAS G, PARIN U, et al. Molecular detection of feline and canine periodontal pathogens[J]. Veterinary and Animal Science, 2019, 8:100069. doi: 10.1016/j.vas.2019.100069
[2]	PAVLICA Z, PETELIN M, JUNTES P, et al. Periodontal disease burden and pathological changes in organs of dogs[J]. Journal of Veterinary Dentistry, 2008, 25(2):97-105. doi: 10.1177/089875640802500210 pmid: 18751659
[3]	SCANNAPIECO F A. Systemic effects of periodontal diseases[J]. Dental Clinics of North America, 2005, 49(3):533-550. pmid: 15978240
[4]	NIEMIEC B A. Periodontal disease[J]. Topics in Companion Animal Medicine, 2008, 23(2):72-80. doi: 10.1053/j.tcam.2008.02.003 pmid: 18482707
[5]	JAYARAM P, CHATTERJEE A, RAGHUNATHAN V. Probiotics in the treatment of periodontal disease: A systematic review[J]. Journal of Indian Society of Periodontology, 2016, 20(5):488-495. doi: 10.4103/0972-124X.207053 pmid: 29242683
[6]	张环, 尹利娟, 陆瑜, 等. 益生菌在口腔中的应用研究[J]. 口腔护理用品工业, 2021, 31(2):18-20.
[7]	中华人民共和国国家市场监督管理总局. 实验动物配合饲料卫生标准:GB/T 14924.2—2001[S]. 北京: 中国标准出版社, 2002.
[8]	中华人民共和国国家市场监督管理总局. 实验动物配合饲料营养成分:GB/T 14924.3—2010[S]. 北京: 中国标准出版社, 2011.
[9]	TURESKY S, GILMORE N D, GLICKMAN I. Reduced plaque formation by the chloromethyl analogue of victamine C[J]. Journal of Periodontology, 1970, 41(1):41-43. doi: 10.1902/jop.1970.41.41.41 pmid: 5264376
[10]	HARVEY C E. Shape and size of teeth of dogs and cats-relevance to studies of plaque and calculus accumulation[J]. Journal of Veterinary Dentistry, 2002, 19(4):186-195. pmid: 12593095
[11]	HARRISON C. Nutrition and preventative oral healthcare treatments for canine and feline patients[J]. The Veterinary Nurse, 2017, 8(8):432-440. doi: 10.12968/vetn.2017.8.8.432
[12]	梁永春, 王小平. 宠物犬牙周病的诊治[J]. 动物医学进展, 2009, 30(7):127-128.
[13]	高飞, 尹航. 犬牙周疾病的综合防治[J]. 中国工作犬业, 2021(6):54-55.
[14]	邹晓娟, 刘建坤, 李冰. 犬牙结石的形成与防治[J]. 中国工作犬业, 2011(4):38.
[15]	顾欢欢, 陈金发, 汪文浩, 等. 洁齿骨对犬牙菌斑和牙结石的影响研究[J]. 中国动物保健, 2021, 23(10):101-102.

益生菌牙膏缓解犬牙菌斑和牙结石的研究

Efficacy Evaluation of a Probiotic Toothpaste in Relieving Canine Dental Plaque and Calculus

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 12

参考文献 15

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘凯莉, 罗建威, 岳珂, 陈盼, 何彦锋, 解静菲, 徐婷婷, 林露茜, 黄淑成. 益生菌在犬猫上的应用研究进展[J]. 畜牧与饲料科学, 2022, 43(3): 46-52.
[2]	姜西迪, 阮崇美, 刘灿, 徐艳丽, 蒲越, 张佳佳, 张家龙, 熊永洁, 李静. 去势术对雄性中华田园犬脂代谢的影响[J]. 畜牧与饲料科学, 2021, 42(6): 112-114.
[3]	姜西迪, 阮崇美, 刘灿, 徐艳丽, 蒲越, 熊永洁, 李静. 去势对公犬血清抗氧化指标的影响[J]. 畜牧与饲料科学, 2021, 42(5): 120-123.
[4]	周松涛, 唐爱民, 冀少波, 陈宝江. 螺旋藻粉和山药粉共同添加对幼犬增重、养分表观消化率和粪便质量的影响[J]. 畜牧与饲料科学, 2021, 42(4): 25-28.
[5]	戴小华, 邵文华, 吴星霖, 王菊梅, 吾买尔江·牙合甫, 郭庆勇, 赵景娣. 某动物医院2017—2018年犬病用药情况分析[J]. 畜牧与饲料科学, 2021, 42(3): 115-122.
[6]	弓玲玲, 卢爱桃, 乌日山, 王瑞, 马蕊, 余永丽, 黄伟, 杜山. 犬人造胃瘘管手术案例分析[J]. 畜牧与饲料科学, 2021, 42(2): 114-116.
[7]	张佳韧, 倪士明, 王瑛琪. 2例犬皮肤肥大细胞瘤细胞学与病理组织学观察[J]. 畜牧与饲料科学, 2021, 42(1): 103-107.
[8]	朱丽秀, 赵建伟, 刘文强, 田思雨, 孙志宇, 张新宇. 山东聊城地区规模化养猪场中重要病毒性传染病的流行情况调查与分析[J]. 畜牧与饲料科学, 2020, 41(3): 92-95.
[9]	王礼伟, 周刚, 王萍, 李鹏. 猪伪狂犬病野毒感染的临床诊断、血清学调查以及控制措施[J]. 畜牧与饲料科学, 2020, 41(3): 96-101.
[10]	魏金凤, 侯蕾蕾, 杨康利, 王进产, 李文龙, 王延辉. 无血清悬浮培养BHK21细胞增殖伪狂犬病毒的参数研究[J]. 畜牧与饲料科学, 2019, 40(9): 6-10.
[11]	胡文举, 宋艳画. 犬精液冷冻保存技术的研究进展[J]. 畜牧与饲料科学, 2019, 40(8): 53-57.
[12]	彭华, 李淑红, 聂佳伟, 郭泽婷. 犬脓皮病病原菌的分离鉴定及耐药性分析[J]. 畜牧与饲料科学, 2019, 40(8): 104-106.
[13]	王峥，朱婷婷，魏雪，韦人月，阮红日，仇爽，聂静，郑家三. 1例犬肱骨外髁骨折的病例报告[J]. 畜牧与饲料科学, 2019, 40(7): 98-100.
[14]	魏金凤, 杨康利, 徐征, 侯蕾蕾, 王延辉. 伪狂犬病毒囊膜糖蛋白gC的研究进展[J]. 畜牧与饲料科学, 2019, 40(7): 101-104.
[15]	赵宝宝, 王宇. 1例犬畸胎瘤的诊疗报告[J]. 畜牧与饲料科学, 2019, 40(7): 105-108.